A novel plasmid carrying blaCTX-M-15 identified in commensal Escherichia coli from healthy pregnant women in Ibadan, Nigeria. Issue 1 (March 2015)

• Record Type: Journal Article
• Authors: Fortini, Daniela; Fashae, Kayode; Villa, Laura; Feudi, Claudia; García-Fernández, Aurora; Carattoli, Alessandra
• Is Part Of: Journal of global antimicrobial resistance. Volume 3:Issue 1(2015:Mar.)
• Page Start: 9
• Page End: 12
• Publication Date: 2015-03
• Subjects: Plasmid-mediated quinolone resistance -- PMQR -- IncF plasmid; Drug resistance -- Periodicals; Drug resistance -- Periodicals; Drug resistance; Periodicals; 616.9041
• DOI: 10.1016/j.jgar.2014.12.002
• Languages: English
• ISSNs: 2213-7165

Abstract

Highlights: We collected 101 faecal samples from healthy women on the day of entrance to the hospital in Ibadan, Nigeria. Samples were screened for commensal Escherichia coli on cefotaxime plates. CTX-M-15 producers were typed. A novel plasmid carrying the bla CTX-M-15, bla TEM-1 and qnrS1 genes was identified and fully sequenced. A PCR screening was devised to search for the novel plasmid in the collection of isolates. Abstract: The aim of this study was to investigate the molecular characteristics of commensal Escherichia coli producing extended-spectrum β-lactamases and showing fluoroquinolone resistance circulating in a healthy population in Ibadan, Nigeria. In total, 101 faecal samples from healthy pregnant women on the day of admission to hospital were collected and plated on eosin–methylene blue agar supplemented with cefotaxime. Genotyping demonstrated the presence of the bla CTX-M-15 gene in all of the cefotaxime-resistant isolates ( n = 32), and there was circulation of prevalent clones. The aac(6′)-Ib -cr, qnrS1, qepA1 and qnrB1 genes were identified in several strains. A novel plasmid supporting the spread of the bla CTX-M-15, bla TEM-1 and qnrS1 genes was identified in these isolates by complete DNA sequencing.