Characterization of a microalgal mutant for CO2 biofixation and biofuel production. (15th August 2016)

• Record Type: Journal Article
• Authors: Qi, Feng; Pei, Haiyan; Hu, Wenrong; Mu, Ruimin; Zhang, Shuo
• Is Part Of: Energy conversion and management. Volume 122(2016)
• Page Start: 344
• Page End: 349
• Publication Date: 2016-08-15
• Subjects: Modified UV mutagenesis -- Chlorella vulgaris -- High CO2 tolerance -- Light conversion efficiency -- Carbohydrates; Direct energy conversion -- Periodicals; Energy storage -- Periodicals; Energy transfer -- Periodicals; Énergie -- Conversion directe -- Périodiques; Direct energy conversion; Periodicals; 621.3105
• DOI: 10.1016/j.enconman.2016.05.093
• Languages: English
• ISSNs: 0196-8904

Abstract

Highlights: Combination of the isolation using 96-well microplates and traditional UV mutagenesis for screening HCT mutant. Microalgal mutant Chlorella vulgaris SDEC-3M was screened out by modified UV mutagenesis. SDEC-3M showed high CO2 tolerance, high CO2 requiring and relevant genetic stability. LCE and carbohydrate content of SDEC-3M were significantly elevated. SDEC-3M offers a strong candidature as CO2 biofixation and biofuel production. Abstract: In the present work, a Chlorella vulgaris mutant, named as SDEC-3M, was screened out through the combination of the isolation using 96-well microplates and traditional UV mutagenesis. Compared with its parent (wild type), the growth of SDEC-3M preferred higher CO2 (15% v/v) environment to ambient air (0.038% CO2 (v/v)), indicating that the mutant qualified with good tolerance and growth potential under high level CO2 (high CO2 tolerance) but was defective in directly utilizing the low level CO2 (high CO2 requiring). The genetic stability under ambient air and high level CO2 was confirmed by a continuous cultivation for five generations. Higher light conversion efficiency (14.52%) and richer total carbohydrate content (42.48%) demonstrated that both solar energy and CO2 were more effectively productively fixed into carbohydrates for bioethanol production than the parent strain. The mutant would benefit CO2 biofixation from industrial exhaust gas to mitigate of global warming and promote biofuel production to relieve energy shortage.