Application of restriction fragment length polymorphism (RFLP) and high resolution melting (HRM) analysis of beta-tubulin gene for species and strains differentiation in Trichophyton mentagrophytes species complex. Issue 3 (September 2016)

• Record Type: Journal Article
• Authors: Sun, Pei-Lun; Lin, Hsin-Chieh; Hsiao, Pa-Fan; Lu, Jang-Jih
• Is Part Of: Journal of dermatological science. Volume 83:Issue 3(2016:Sep.)
• Page Start: 247
• Page End: 250
• Publication Date: 2016-09
• Subjects: Restriction fragment length polymorphism -- High resolution melting -- Trichophyton interdigitale -- Anthropophilic -- Zoophilic -- Dermatophytes; Dermatology -- Periodicals; Skin Diseases -- Periodicals; Dermatologie -- Périodiques; 616.5005
• DOI: 10.1016/j.jdermsci.2016.05.013
• Languages: English
• ISSNs: 0923-1811

Abstract

Highlights: Anthropophilic and zoophilic strain of T. interdigitale are morphologically identical. Precise identification of these two strains is clinically important. Partial beta-tubulin gene is an ideal region for strain differentiation. Combined RFLP and HRM analyses of partial beta-tubulin gene can discriminate these two strains.