The Myb domain of LUX ARRHYTHMO in complex with DNA: expression, purification and crystallization. Issue 5 (1st May 2016)

• Record Type: Journal Article
• Authors: Silva, Catarina S.; Lai, Xuelei; Nanao, Max; Zubieta, Chloe
• Is Part Of: Acta crystallographica. Volume 72:Issue 5(2016:May)
• Page Start: 356
• Page End: 361
• Publication Date: 2016-05-01
• Subjects: Myb domain -- DNA binding -- protein–DNA complex -- LUX ARRHYTHMO -- Arabidopsis thaliana; Crystallography -- Periodicals; Crystals -- Periodicals; 548
• DOI: 10.1107/S2053230X16004684
• Languages: English
• ISSNs: 2053-230X

Abstract

Abstract : The expression, purification and crystallization of the DNA‐binding Myb domain of LUX ARRHYTHMO from A. thaliana in complex with DNA are reported. The binding affinity of the Myb domain for DNA was measured and was found to be in the low‐nanomolar range, suggesting that a single Myb domain is able to bind to its cognate DNA with very high affinity. Abstract : LUX ARRHYTHMO (LUX) is a Myb‐domain transcription factor that plays an important role in regulating the circadian clock. Lux mutations cause severe clock defects and arrhythmia in constant light and dark. In order to examine the molecular mechanisms underlying the function of LUX, the DNA‐binding Myb domain was cloned, expressed and purified. The DNA‐binding activity of the Myb domain was confirmed using electrophoretic mobility shift assays (EMSAs), demonstrating that the LUX Myb domain is able to bind to DNA with nanomolar affinity. In order to investigate the specificity determinants of protein–DNA interactions, the protein was co‐crystallized with a 10‐mer cognate DNA. Initial crystallization results for the selenomethionine‐derivatized protein and data‐set collection statistics are reported. Data collection was performed using the MeshAndCollect workflow available at the ESRF.