NF‐κB‐dependent signals control BOB.1/OBF.1 and Oct2 transcriptional activity in B cells. Issue 12 (20th October 2015)

• Record Type: Journal Article
• Authors: Kilzheimer, Melanie; Quandt, Jasmin; Langhans, Julia; Weihrich, Petra; Wirth, Thomas; Brunner, Cornelia
• Is Part Of: European journal of immunology. Volume 45:Issue 12(2015:Dec.)
• Page Start: 3441
• Page End: 3453
• Publication Date: 2015-10-20
• Subjects: B cells BOB.1/OBF.1 (Pou2af1) Oct2 (Pou2f2) promoter analyses transcriptional regulation; Immunology -- Periodicals; 616.079
• DOI: 10.1002/eji.201545475
• Languages: English
• ISSNs: 0014-2980

Abstract

Abstract : The transcriptional co‐activator BOB.1/OBF.1 is crucial for Octamer‐driven transcription in B cells. BOB.1/OBF.1‐deficiency leads to tremendous defects in B‐cell development and function. Therefore, in the past research focused on the identification of BOB.1/OBF.1 target genes. However, the regulation of BOB.1/OBF.1 expression itself is poorly understood. Here we show that in B cells NF‐κB as well as to some extent NFAT proteins are involved in the activation of basal as well as inducible BOB.1/OBF.1 expression by direct binding to the BOB.1/OBF.1 promoter. Moreover, the analysis of different inducers of NF‐κB, like several TLR ligands, TNF‐α, BAFF, or LTα1β2, revealed that both the canonical and noncanonical NF‐κB pathways are involved in the induction of BOB.1/OBF.1 gene. The identification of so far unknown inducers that regulate BOB.1/OBF.1 expression in B cells provides novel insights in the potential function of BOB.1/OBF.1 during different aspects of B‐cell development and function.