Pulmonary Chlamydia muridarum challenge activates lung interstitial macrophages which correlate with IFN‐γ production and infection control in mice. Issue 12 (28th September 2015)

• Record Type: Journal Article
• Authors: Gracey, Eric; Baglaenko, Yuriy; Prayitno, Nadia; Van Rooijen, Nico; Akram, Ali; Lin, Aifeng; Chiu, Basil; Inman, Robert D.
• Is Part Of: European journal of immunology. Volume 45:Issue 12(2015:Dec.)
• Page Start: 3417
• Page End: 3430
• Publication Date: 2015-09-28
• Subjects: Chlamydia -- IFN‐γ -- Infection -- Lung -- Macrophage; Immunology -- Periodicals; 616.079
• DOI: 10.1002/eji.201545874
• Languages: English
• ISSNs: 0014-2980

Abstract

Abstract : Protective immunity to the pathogen Chlamydia is dependent on a robust IFN‐γ response generated by innate and adaptive lymphocytes. Here we assess the role of the macrophage in orchestrating a protective response in vivo to the murine pathogen, Chlamydia muridarum . During acute pulmonary and peritoneal infection, resident macrophages in both sites are infected with C. muridarum and adopt an inflammatory phenotype. In the lung, this activation is restricted to interstitial macrophages, which harbor higher levels of C. muridarum 16sRNA than alveolar macrophages. We examined innate and adaptive lymphocyte activation in the peritoneal cavity with macrophage depletion and with adoptive transfer of infected macrophages. These experiments demonstrate macrophage activation correlates with a protective IFN‐γ response and effective control of C. muridarum . These studies suggest that a quantitative or qualitative alteration in macrophages may play a key role in the development of Chlamydia ‐associated diseases.