Tungsten disulfide Quantum Dots Based Disposable Paper Based Lab on GenoChip for Specific Meningitis DNA Detection. Issue 10 (28th May 2020)

• Record Type: Journal Article
• Authors: Gupta, Riya; Ozhukil Valappil, Manila; Sakthivel, Arunkumar; Mathur, Ashish; Pundir, Chandra Shekhar; Murugavel, Kathiresan; Narang, Jagriti; Alwarappan, Subbiah
• Is Part Of: Journal of the Electrochemical Society. Volume 167:Issue 10(2020)
• Publication Date: 2020-05-28
• Subjects: Paper-based analytical device (PAD) -- Bacterial Meningitits -- Electrochemical Genosensor -- Tungsten disulfide quantum dots (WS2QDs) -- DNA Sensors; Electrochemistry -- Periodicals; 541.3705
• DOI: 10.1149/1945-7111/ab8fda
• Languages: English
• ISSNs: 0013-4651

Abstract

Abstract : Tungsten disulfide quantum dots (WS2 QDs) embedded lab on genochip based analytical device (PAD) was developed for the specific DNA detection of Meningitis, a life threatening disease. This paper-based lab on genochip was designed by grafting the two electrodes onto the surface of paper using conductive ink. The WS2 QDs were characterized by X-ray diffraction technique, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Following this, specific oligonucleotide sequence of DNA (capture probe) was immobilized over the surface of WS2 QDs/PAD electrode. The ssDNA/WS2 QDs/PAD device was characterized electrochemically by cyclic voltammetry. The device was employed for detection of target DNA (by hybridization) by employing methylene blue (MB) as an indicator. The biosensor exhibited a wide linear response in the range 1 nM–100 μM towards the target DNA. The limit of detection (LOD) of target DNA of the proposed device is 1 nM. The advantageous features of this device than the existing biosensors include cheaper analysis cost, tiny sample requirements and stability.