Investigation of the protective effect of nesfatin‐1 on testicular ischaemia‐reperfusion damage: An experimental study. (16th August 2020)

• Record Type: Journal Article
• Authors: Erdogan, Omer; Çetinkaya, Mehmet; Sahin, Hayrettin; Deliktas, Hasan; Dere, Yelda; Yılmaz, Mustafa; Tosun, Kursad; Aktas, Serdar
• Is Part Of: Andrologia. Volume 52:Number 10(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-08-16
• Subjects: ischaemia -- nesfatin‐1 -- oxidative stress -- reperfusion -- testis torsion; Andrology -- Periodicals; Infertility, Male -- Periodicals; 616.69
• DOI: 10.1111/and.13769
• Languages: English
• ISSNs: 0303-4569

Abstract

Abstract: This study aimed to determine oxidative stress in the tissue after testicular torsion biochemically and histopathologically and to examine the effects of Nesfatin‐1 treatment on this injury. Thirty‐two rats were randomly divided into four groups: sham, torsion + detorsion (4 hr torsion followed by 1 hr detorsion), ischaemia/reperfusion + saline (I/R + S) and I/R + nesfatin‐1. I/R + S group a single‐dose saline treatment was administered intraperitoneally at the two‐hundred‐tenth minute of torsion (ischaemia; 10 cc/kg). Similarly, I/R + nesfatin‐1 group a single dose of nesfatin‐1 treatment was administered intraperitoneally at the two‐hundred‐tenth minute of ischaemia (10 µg/kg). Myeloperoxidase, total oxidant status and oxidative stress index values were significantly increased in the I/R and I/R + S group compared to the sham group. Superoxide dismutase was significantly decreased in the I/R + S group compared to the sham group. No significant difference was found between the I/R + nesfatin‐1 group and the other I/R groups (I/R and I/R + S) in terms of biochemical parameters. The mean diameter of the seminiferous tubule decreased in the I/R groups. However, the mean diameter of the seminiferous tubules was not significantly different between the I/R + S group and the I/R + nesfatin‐1 group. Thus, the administration of nesfatin‐1 after ischaemia did not reduce testicular‐oxidative stress.