Characterization of ancestral myosin XI from Marchantia polymorpha by heterologous expression in Arabidopsis thaliana. (6th August 2020)

• Record Type: Journal Article
• Authors: Duan, Zhongrui; Tanaka, Misato; Kanazawa, Takehiko; Haraguchi, Takeshi; Takyu, Akiko; Era, Atsuko; Ueda, Takashi; Ito, Kohji; Tominaga, Motoki
• Is Part Of: Plant journal. Volume 104:Number 2(2020)
• Page Start: 460
• Page End: 473
• Publication Date: 2020-08-06
• Subjects: cytoplasmic streaming -- Marchantia polymorpha -- myosin XI -- heterologous expression; Plant molecular biology -- Periodicals; Plant cells and tissues -- Periodicals; Botany -- Periodicals; 580
• DOI: 10.1111/tpj.14937
• Languages: English
• ISSNs: 0960-7412

Abstract

Summary: Previous studies have revealed duplications and diversification of myosin XI genes between angiosperms and bryophytes; however, the functional differentiation and conservation of myosin XI between them remain unclear. Here, we identified a single myosin XI gene from the liverwort Marchantia polymorpha (Mp). The molecular properties of Mp myosin XI are similar to those of Arabidopsis myosin XIs responsible for cytoplasmic streaming, suggesting that the motor function of myosin XI is able to generate cytoplasmic streaming. In cultured Arabidopsis cells, transiently expressed green fluorescent protein (GFP)‐fused Mp myosin XI was observed as some intracellular structures moving along the F‐actin. These intracellular structures were co‐localized with motile endoplasmic reticulum (ER) strands, suggesting that Mp myosin XI binds to the ER and generates intracellular transport in Arabidopsis cells. The tail domain of Mp myosin XI was co‐localized with that of Arabidopsis myosin XI‐2 and XI‐K, suggesting that all these myosin XIs bind to common cargoes. Furthermore, expression of GFP‐fused Mp myosin XI rescued the defects of growth, cytoplasmic streaming and actin organization in Arabidopsis multiple myosin XI knockout mutants. The heterologous expression experiments demonstrated the cellular and physiological competence of Mp myosin XI in Arabidopsis. However, the average velocity of organelle transport in Marchantia rhizoids was 0.04 ± 0.01 μm s −1, which is approximately one‐hundredth of that in Arabidopsis cells. Taken together, our results suggest that the molecular properties of myosin XI are conserved, but myosin XI‐driven intracellular transport in vivo would be differentiated from bryophytes to angiosperms. Significance Statement: The molecular properties and heterologous expression of Marchantia polymorpha myosin XI in Arabidopsis suggest that myosin XI is an evolutionarily conserved motor protein capable of generating cytoplasmic streaming.