A Novel Hybrid Silk Fibroin/Polyurethane Arteriovenous Graft for Hemodialysis: Proof‐of‐Concept Animal Study in an Ovine Model. Issue 20 (11th September 2020)

• Record Type: Journal Article
• Authors: Riboldi, Stefania Adele; Tozzi, Matteo; Bagardi, Mara; Ravasio, Giuliano; Cigalino, Giorgio; Crippa, Luca; Piccolo, Solange; Nahal, Amal; Spandri, Mattia; Catto, Valentina; Tironi, Matteo; Greco, Francesco Giovanni; Remuzzi, Andrea; Acocella, Fabio
• Is Part Of: Advanced healthcare materials. Volume 9:Issue 20(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-09-11
• Subjects: hemodialysis -- hybrid vascular grafts -- ovine models -- silk fibroin -- vascular access; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/adhm.202000794
• Languages: English
• ISSNs: 2192-2640

Abstract

Abstract: To solve the problem of vascular access failure, a novel semi‐degradable hybrid vascular graft, manufactured by electrospinning using silk fibroin and polyurethane (Silkothane), has been previously developed and characterized in vitro. This proof‐of‐concept animal study aims at evaluating the performances of Silkothane grafts in a sheep model of arteriovenous shunt, in terms of patency and short‐term remodeling. Nine Silkothane grafts are implanted between the common carotid artery and the external jugular vein of nine sheep, examined by palpation three times per week, by echo‐color Doppler every two weeks, and euthanized at 30, 60, and 90 days ( N = 3 per group). At sacrifice, grafts are harvested and submitted for histopathology and/or scanning electron microcopy (SEM). No cases of graft‐related complications are recorded. Eight of nine sheep (89%) show 100% primary unassisted patency at the respective time of sacrifice (flow rate 1.76 ± 0.61 L min −1, one case of surgery‐related thrombosis excluded). Histopathology and SEM analysis evidence signs of inflammation and pseudointima inside the graft lumen, especially at the venous anastomosis; however, endoluminal stenosis never impairs the functionality of the shunt and coverage by endothelial cells is observed. In this model, Silkothane grafts grant safety and 100% patency up to 90 days. Abstract : A novel hybrid vascular graft, manufactured by electrospinning using silk fibroin and polyurethane, is tested herein in an ovine model of carotid‐jugular arteriovenous shunt, providing evidences of safety and efficacy in granting 100% patency up to 90 days. The graft can potentially be employed as vascular access for hemodialysis, as well as for revascularization procedures.