Characterization and Proteomic Analysis of Decellularized Adipose Tissue Hydrogels Derived from Lean and Overweight/Obese Human Donors. Issue 10 (11th September 2020)

• Record Type: Journal Article
• Authors: Mohiuddin, Omair A.; Motherwell, Jessica M.; Rogers, Emma; Bratton, Melyssa R.; Zhang, Qiang; Wang, Guangdi; Bunnell, Bruce; Hayes, Daniel J.; Gimble, Jeffrey M.
• Is Part Of: Advanced biosystems. Volume 4:Issue 10(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-09-11
• Subjects: adipose stem cells (ASC) -- biomaterials -- decellularized adipose tissue hydrogel -- liquid chromatography mass‐spectrometry (LC‐MS) -- proteomics; Biological systems -- Periodicals; Biotechnology -- Periodicals; Bioengineering -- Periodicals; Biomedical engineering -- Periodicals; Biological Science Disciplines; Periodicals; Periodicals; 660.6
• DOI: 10.1002/adbi.202000124
• Languages: English
• ISSNs: 2366-7478

Abstract

Abstract: While decellularized adipose tissue (DAT) has potential as an "off‐the‐shelf" biomaterial product for regenerative medicine, it remains to be determined if donor‐source body mass index (BMI) impacts the functionality of DAT. This study set out to comparatively characterize lean versus overweight/obese‐donor derived DAT hydrogel based on proteome and to analyze their respective effects on adipose stromal/stem cell (ASC) viability, and differentiation in vitro. Decellularized adipose tissue from lean (lDAT) and overweight/obese (oDAT) donors is produced and characterized. Variability in the fibril microstructures is found, with dense fibrotic fiber clusters and large pore area uniquely present in the oDAT samples. Proteomic analysis reveals that lDAT contains a greater proportion of enriched extracellular proteins and a smaller proportion of enriched intracellular proteins relative to oDAT. Biocompatibility studies show that ASCs cultured in lDAT and oDAT hydrogels remain viable. The adipogenic and osteogenic differentiation capability of ASCs seeded in lDAT and oDAT hydrogels is confirmed by an upregulation in marker gene expression and phenotypic analysis. In conclusion, this study establishes that DAT hydrogels derived from lean and overweight/obese adipose donors present similar physicochemical profiles with some distinctive features while comparably supporting the viability and adipogenic differentiation of ASCs in vitro. Abstract : Decellularized fat tissue has potential as a regenerative medical product for repair of soft tissue cosmetic and reconstructive defects. This study evaluates the protein composition and cellular biocompatibility of decellularized fat hydrogels prepared from either lean or overweight/obese tissue donors. While the majority of proteins are similar between groups, lean donor hydrogels show a relative enrichment of extracellular matrix proteins.