A novel NIR fluorescent probe for fast detection and imaging of methionine sulfoxide reductase A in lysosome of living cells. Issue 7 (21st September 2020)

• Record Type: Journal Article
• Authors: Gong, Jin; Liu, Chang; Cai, Songtao; He, Song; Zhao, Liancheng; Zeng, Xianshun
• Is Part Of: Materials advances. Volume 1:Issue 7(2020)
• Page Start: 2401
• Page End: 2406
• Publication Date: 2020-09-21
• Subjects: 620.11
• DOI: 10.1039/d0ma00489h
• Languages: English
• ISSNs: 2633-5409

Abstract

Abstract : A novel lysosome-targeted NIR fluorescent probe for fast detection and imaging Msr A was developed by extending the linear π-conjugated system of the probe via SN Ar H reaction. Abstract : Methionine sulfoxide reductases (Msrs) play special roles in protecting proteins from oxidative damage caused by reactive species and repairing MetSO residues in proteins/peptides. However, current probes for Msrs fail to target lysosomes and exhibit a relatively slow response and short emission wavelengths (less than 580 nm). Herein, we reported a novel lysosome-targeted NIR fluorescent probe RBSO for fast detection and imaging Msr A by extending the linear π-conjugated system of the probe via the SN Ar H reaction. As expected, RBSO exhibited high selectivity and high sensitivity for the rapid detection of Msr A in real-time via a specific Msr A-triggered methylsulfinyl reduction reaction. Importantly, RBSO was cell-membrane permeable and capable of specifically distributing in lysosomes, and thus detects Msr A in real-time there, providing a potential tool for monitoring Msr A activity in lysosomes of living cells.