Non-photosynthetic CO2 bio-mitigation by Escherichia coli harbouring CBB genes. Issue 20 (28th September 2020)

• Record Type: Journal Article
• Authors: Lee, Soo Youn; Kim, Young Su; Shin, Woo-Ri; Yu, Jaeyoung; Lee, Jiye; Lee, Sangmin; Kim, Yang-Hoon; Min, Jiho
• Is Part Of: Green chemistry. Volume 22:Issue 20(2020)
• Page Start: 6889
• Page End: 6896
• Publication Date: 2020-09-28
• Subjects: Environmental chemistry -- Industrial applications -- Periodicals; Environmental management -- Periodicals; 660
• DOI: 10.1039/d0gc01820a
• Languages: English
• ISSNs: 1463-9262

Abstract

Abstract : To alleviate carbon dioxide (CO2 ) emission, alternative approaches have been considered, such as employing microorganisms capable of CO2 -fixation using their own metabolic pathways. Abstract : To alleviate carbon dioxide (CO2 ) emission, alternative approaches have been considered, such as employing microorganisms capable of CO2 -fixation using their own metabolic pathways. In this study, we introduced a synthetic CO2 -fixation pathway into the industrial platform microorganism Escherichia coli to achieve immediate CO2 mitigation with a non-photosynthetic reaction. Specifically, the whole gene clusters ( cbb I and cbb II operons) involved in the Calvin–Benson–Bassham (CBB) pathway in Rhodobacter sphaeroides (rCBB) were expressed heterogeneously in E. coli under a restrictive carbon source and dark conditions. The resulting strain with the whole rCBB was able to exhibit a higher level of endogenous CO2 recycling compared to the control strain. In addition, the electron requirements from the cathode and redox cofactors were increased following the activation of the CO2 -fixation pathway without light irradiation. Exogenous CO2 from yeast fermentation was also mitigated through a simple gas-phase connection with the E. coli /rCBB culture system. This bacterial CO2 mitigation strategy has great potential to alleviate greenhouse gas emissions through biological processes to produce carbon neutral biofuels and value-added chemicals.