CRISPR-Cas12a trans-cleaves DNA G-quadruplexes. Issue 83 (23rd September 2020)

• Record Type: Journal Article
• Authors: Li, Ying; Li, Tao; Liu, Bi-Feng; Hu, Rui; Zhu, Jiang; He, Ting; Zhou, Xin; Li, Conggang; Yang, Yunhuang; Liu, Maili
• Is Part Of: Chemical communications. Volume 56:Issue 83(2020)
• Page Start: 12526
• Page End: 12529
• Publication Date: 2020-09-23
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d0cc05540a
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : CRISPR-Cas12a trans -cleaves DNA G-quadruplexes after activation. Abstract : We for the first time report that the activated CRISPR-Cas12a system trans -cleaves DNA G-quadruplexes (G4). The cleavage activity on human telomere G4 and TBA G4 was investigated and verified by FRET, CD, gel electrophoresis and NMR. We believe that this finding will pave a new avenue for advancing the applications of CRISPR-Cas12a and G4 in biosensing and biochemistry.