Expression profile analysis of circular RNAs in BmN cells (Bombyx mori) upon BmNPV infection. Issue 3 (2nd September 2020)

• Record Type: Journal Article
• Authors: Zhang, Shaolun; Shen, Manman; Yin, Haotong; Huang, Haoling; Li, Tao; Zhao, Weiguo; Guo, Xijie; Wu, Ping
• Is Part Of: Archives of insect biochemistry and physiology. Volume 105:Issue 3(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-09-02
• Subjects: circular RNA -- competing endogenous RNAs -- host–pathogen interaction -- nucleopolyhedrovirus -- silkworm; Insects -- Physiology -- Periodicals; Insect biochemistry -- Periodicals; 595.701572
• DOI: 10.1002/arch.21735
• Languages: English
• ISSNs: 0739-4462

Abstract

Abstract: The disease caused by Bombyx mori nucleopolyhedrovirus (BmNPV) has always been difficult to control, resulting in tremendous economic losses in the sericulture industry. Although much has been learned about the impact of noncoding RNAs on pathogenesis, the role of circular RNA (circRNA) in insect immunity remains unclear. To explore circRNA regulation involved in BmNPV infection, we used transcriptome analysis of BmN cells with or without BmNPV infection to generate circRNA data set. A total of 444 novel circRNAs were identified in BmN cells, with 198 pervasively distributed both in the control group and BmNPV‐infection group. The host genes were enriched inMAPK signaling pathway, dorso–ventral axis formation, and ECM–receptor interaction, which were required for the normal larval growth. A total of 75 circRNAs were differentially expressed (DE) on BmNPV infection. Six downregulated circRNAs were validated by Sanger sequencing and qRT‐PCR. DEcircRNA–miRNA–DEmRNA network was constructed based on the six validated circRNAs. Pathway analysis indicated that the predicted target genes were mainly enriched in the metabolic pathway and immune‐related signaling pathway. Our results may provide a basis for further studies on circRNA function in BmN cells challenged by BmNPV infection and offer an insight into the molecular mechanism on silkworm–virus interaction. Abstract : Expression profile analysis of circular RNAs (circRNAs) in BmN cells ( Bombyx mori ) upon BmNPV infection. (a) Heatmap representing the log10(TPM) of all differentially expressed circRNAs; (b) competing endogenous RNA regulatory network; and (c) validation of differentially expressed circRNAs. Research Highlights: A total of 444 novel circRNAs identification in BmN cells on BmNPV infection, among which 75 circRNAs were differentially expressed. Metabolic pathway and immune‐related signaling pathway were enriched for predicted genes in ceRNA analysis.