Chemical characterisation and quantification of the major constituents in the Chinese herbal formula Jian‐Pi‐Yi‐Shen pill by UPLC‐Q‐TOF‐MS/MS and HPLC‐QQQ‐MS/MS. Issue 6 (3rd June 2020)

• Record Type: Journal Article
• Authors: Wang, Fochang; Huang, Shiying; Chen, Qiugu; Hu, Zhaoliu; Li, Zhonggui; Zheng, Ping; Liu, Xinhui; Li, Shunmin; Zhang, Shangbin; Chen, Jianping
• Is Part Of: Phytochemical analysis. Volume 31:Issue 6(2020)
• Page Start: 915
• Page End: 929
• Publication Date: 2020-06-03
• Subjects: HPLC‐QQQ‐MS/MS -- Jian‐Pi‐Yi‐Shen pill -- qualitative and quantitative analysis -- UPLC‐Q‐TOF‐MS/MS; Plants -- Analysis -- Periodicals; Plants -- chemistry -- Periodicals; 572.2
• DOI: 10.1002/pca.2963
• Languages: English
• ISSNs: 0958-0344

Abstract

Abstract: Introduction: Jian‐Pi‐Yi‐Shen pill (JPYSP) is a Chinese medicine formula developed for the treatment of anaemic patients with chronic kidney disease (CKD). Objective: To investigate the chemical profile of JPYSP in the treatment of renal anaemia. Methods: A method coupling ultra‐performance liquid chromatography with quadrupole time‐of‐flight tandem mass spectrometry (UPLC‐Q‐TOF‐MS/MS) was established to characterise the chemical constituents present in JPYSP. Subsequently, a high‐performance liquid chromatography method coupled with triple‐quadrupole tandem mass spectrometry (HPLC‐QQQ‐MS/MS) was developed to quantify the major constituents from the identified compounds related to the treatment of CKD and anaemia. Results: A total of 71 compounds were tentatively identified from JPYSP, including saponins, flavonoids, sesquiterpenoids, coumarins, phenylpropanoids, anthranones, anthraquinones, tannins, phenolic acids and others. Amongst them, 12 compounds (i.e. astragaloside IV, calycosin, calycosin 7‐ O ‐glucoside, salvianolic acid A, rosmarinic acid, rhein, liquiritin, formononetin, atractylenolide I, dioscin, tanshinone IIA, and acteoside) were further quantified simultaneously by HPLC‐QQQ‐MS/MS. Conclusion: The newly developed approach is suitable for the chemical profiling analysis and quality control of JPYSP, and could lead to additional pharmacodynamic studies involving the components of JPYSP. Abstract : 1. Probing the chemical profiles of JPYSP and seventy‐one components in JPYSP were identified by UPLC‐Q‐TOF‐MS/MS. 2. Twelve components in JPYSP were simultaneously quantified by HPLC‐QQQ‐MS/MS. 3. The fragments patterns of major compounds in JPYSP were summarized for rapid structural speculation.