Transport of nanoprobes in multicellular spheroids. Issue 38 (25th September 2020)

• Record Type: Journal Article
• Authors: Pilz, Marta; Kwapiszewska, Karina; Kalwarczyk, Tomasz; Bubak, Grzegorz; Nowis, Dominika; Hołyst, Robert
• Is Part Of: Nanoscale. Volume 12:Issue 38(2020)
• Page Start: 19880
• Page End: 19887
• Publication Date: 2020-09-25
• Subjects: Nanoscience -- Periodicals; Nanotechnology -- Periodicals; 620.505
• DOI: 10.1039/d0nr01986k
• Languages: English
• ISSNs: 2040-3364

Abstract

Abstract : We present the study of the nanoprobes' diffusion in the extracellular matrix (ECM) of multicellular spheroids. Abstract : The efficient delivery of drugs to cells depends on their diffusion through the extracellular matrix (ECM) of tissues. Here we present a study on the diffusion of nanoprobes of radius from 1 nm to over 100 nm in the ECM of spheroids of three cell types (HeLa, MCF-7 and fibroblasts). We quantified the nanoparticle transport in the spheroids' proliferating zone. We determined the size-dependent viscosity of the ECM. We revealed that nanoobjects up to 10 nm in radius exhibited unobstructed diffusion in the ECM, regardless of the spheroid type. The presented length-scale dependent viscosity profiles for spheroids pave the way for advanced modelling of drug administration through tissues.