Multiomic analysis of a dried single-drop plasma sample using an integrated mass spectrometry approach. Issue 20 (12th August 2020)

• Record Type: Journal Article
• Authors: Gao, Weina; Zhang, Qiaoyun; Su, Yiran; Huang, Peiwu; Lu, Xue; Gong, Qinyue; Chen, Wendong; Xu, Ruilian; Tian, Ruijun
• Is Part Of: Analyst. Volume 145:Issue 20(2020)
• Page Start: 6441
• Page End: 6446
• Publication Date: 2020-08-12
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/d0an01149e
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : An easy-to-use and fast approach was developed for integrated proteomic and metabolic profiling in a dried single-drop plasma sample. Abstract : An easy-to-use and fast approach was developed for integrated proteomic and metabolic profiling in a dried single-drop plasma sample. Plasma collection, room temperature storage, and sample preparation for both proteins and metabolites were seamlessly integrated in one spintip device. MS-based multiomic profiling using the same nano LC-MS system identified more than 150 proteins and 160 metabolites from the 1 μL plasma sample in 6 hours. Further combination with micro-flow LC and targeted MS made it a promising approach for the fast profiling of molecular biomarkers with high sensitivity and accuracy.