O-specific polysaccharide structure isolated from the LPS of the Antarctic bacterium Pseudomonas ANT_J38B. (November 2020)

• Record Type: Journal Article
• Authors: Di Guida, Rossella; Casillo, Angela; Corsaro, Maria Michela
• Is Part Of: Carbohydrate research. Volume 497(2020)
• Publication Date: 2020-11
• Subjects: Lipopolysaccharide -- NMR -- Psychrotolerant -- Pseudomonas -- Gulopyranuronamide; Carbohydrates -- Periodicals; Chemistry, Organic -- Periodicals; Biochemistry -- Periodicals; Carbohydrates -- Periodicals; Chimie organique -- Périodiques; Glucides -- Périodiques; Biochemistry; Carbohydrates; Chemistry, Organic; Periodicals; Electronic journals; 507.78
• DOI: 10.1016/j.carres.2020.108125
• Languages: English
• ISSNs: 0008-6215

Abstract

Abstract: Pseudomonas ANT_J38B is a Gram-negative bacterium isolated from an Antarctic island. LPS was extracted using the phenol/chloroform/petroleum ether method. A mild acid hydrolysis followed by a gel filtration purification afforded the O-chain. The polysaccharide was characterized by means of chemical analyses and NMR spectroscopy. The O-chain displays a disaccharide repeating unit with the following backbone: →4)-α-l -Gul p NAc3OAcAN-(1 →3)-β-d -Qui p NAc-(1→ . Graphical abstract: Image 1 Highlights: The Antarctic bacterium Pseudomonas ANT_J38B produced a smooth-LPS. The O-specific polysaccharide from Pseudomonas ANT_J38B was extracted and purified. The OPS structure was completely characterized by 1D and 2D NMR spectroscopy. The OPS consists of a disaccharide repeating unit containing β-d -Qui2NAc and α-l -GulNAc3OAcAN.