Improving confirmed nanometric sulfur bioproduction using engineered Thioalkalivibrio versutus. (December 2020)

• Record Type: Journal Article
• Authors: Sharshar, Moustafa Mohamed; Samak, Nadia Abdrabou; Ambreen, Sadaf; Hao, Xuemi; Mu, Tingzhen; Maarouf, Mohamed; Zheng, Chen; Gao, Yibo; Liu, Zhixia; Jia, Yunpu; Li, Xiangyuan; Zhong, Wei; Peh, Sumit; Yang, Maohua; Xing, Jianmin
• Is Part Of: Bioresource technology. Volume 317(2020)
• Publication Date: 2020-12
• Subjects: Nanometric sulfur bioproduction -- Desulfurization -- Cell death-induced CRISPR -- Sulfur pathways -- Thioalkalivibrio versutus; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2020.124018
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Nanometric sulfur production (less than 50 nm) from biodesulfurization were proved. Sulfate producing genes were knocked out and its production was declined by 55.1% Nanometric sulfur production was enhanced by 166.7% using sulfide as energy source. Cell death induced – CRISPR system was developed with 42% editing efficiency. Sulfur metabolism was uncovered using protein mining and transcriptome studies. Abstract: Complicated production procedures and superior characteristics of nano-sized sulfur elevate its price to 25–40 fold higher than micrograde kind. Also, natural gas hydrogen sulfide levels are restricted because of its toxic environmental consequences. Thioalkalivibrio versutus is a polyextremophilic industrial autotroph with high natural gas desulfurization capability. Here, nanometric (>50 nm) sulfur bioproduction using T. versutus while desulfurizing natural gas was validated. Also, this production was enhanced by 166.7% via lowering sulfate production by 55.1%. A specially-developed CRISPR system, with 42% editing efficiency, simplified the genome editing workflow scheme for this challenging bacterium. In parallel, sulfur metabolism was uncovered using proteins mining and transcriptome studies for defining sulfate-producing key genes (heterodisulfide reductase-like complex, sulfur dioxygenase, sulfite dehydrogenase and sulfite oxidase). This study provided cost-effective nanometric sulfur production and improved this production using a novel CRISPR strategy, which could be suitable for industrial polyextremophiles, after uncovering sulfur pathways in T. versutus .