Investigation of HMG‐CoA reductase inhibitory and antioxidant effects of various hydroxycoumarin derivatives. Issue 10 (10th July 2020)

• Record Type: Journal Article
• Authors: Ozalp, Lalehan; Danış, Özkan; Yuce‐Dursun, Basak; Demir, Serap; Gündüz, Cihan; Ogan, Ayse
• Is Part Of: Archiv der Pharmazie. Volume 353:Issue 10(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-07-10
• Subjects: antioxidant -- coumarin -- HMG‐CoA reductase -- lipid lowering -- radical scavenging; Pharmaceutical chemistry -- Periodicals; Pharmacology -- Periodicals; 615.19
• DOI: 10.1002/ardp.201900378
• Languages: English
• ISSNs: 0365-6233

Abstract

Abstract: Cardiovascular diseases are one of the primary causes of deaths worldwide, and the development of atherosclerosis is closely related to hypercholesterolemia. As the reduction of the low‐density lipoprotein cholesterol level is critical for treating these diseases, the inhibition of 3‐hydroxy‐3‐methyl‐glutaryl coenzyme A (HMG‐CoA) reductase, which is essentially responsible for cholesterol biosynthesis, stands out as a key solution to lower plasma cholesterol levels. In this study, we synthesized several dihydroxycoumarins and investigated their antioxidant and in vitro HMG‐CoA reductase inhibitory effects. Furthermore, we carried out in silico studies and examined the quantum‐chemical properties of the coumarin derivatives. We also performed molecular docking experiments and analyzed the binding strength of each coumarin derivative. Our results revealed that compound IV displayed the highest HMG‐CoA reductase inhibitory activity (IC50 = 42.0 µM) in vitro. Cupric‐reducing antioxidant capacity and ferric‐reducing antioxidant power assays demonstrated that coumarin derivatives exhibit potent antioxidant activities. Additionally, a close relationship was found between the lowest unoccupied molecular orbital energy levels and the antioxidant activities. Abstract : Five dihydroxycoumarins were synthesized and investigated for their antioxidant and in vitro 3‐hydroxy‐3‐methyl‐glutaryl coenzyme A (HMG‐CoA) reductase inhibitory effects. The quantum‐chemical properties of the coumarin derivatives were explored and molecular docking was performed to determine the binding strength of each coumarin derivative. Compound IV displayed the highest HMG‐CoA reductase inhibitory activity, with IC50 = 42.0 µM.