In vivo clearance of 19F MRI imaging nanocarriers is strongly influenced by nanoparticle ultrastructure. (December 2020)

• Record Type: Journal Article
• Authors: Staal, Alexander H.J.; Becker, Katrin; Tagit, Oya; Koen van Riessen, N.; Koshkina, Olga; Veltien, Andor; Bouvain, Pascal; Cortenbach, Kimberley R.G.; Scheenen, Tom; Flögel, Ulrich; Temme, Sebastian; Srinivas, Mangala
• Is Part Of: Biomaterials. Volume 261(2020)
• Publication Date: 2020-12
• Subjects: Nanoparticle -- Perfluorocarbon -- PFCE -- 19F MRI -- Clearance -- Myocardial infarction -- Cell tracking; Biomedical materials -- Periodicals; Biocompatible Materials -- Periodicals; Biomatériaux -- Périodiques; 610.28
• DOI: 10.1016/j.biomaterials.2020.120307
• Languages: English
• ISSNs: 0142-9612

Abstract

Abstract: Perfluorocarbons hold great promise both as imaging agents, particularly for 19 F MRI, and in therapy, such as oxygen delivery. 19 F MRI is unique in its ability to unambiguously track and quantify a tracer while maintaining anatomic context, and without the use of ionizing radiation. This is particularly well-suited for inflammation imaging and quantitative cell tracking. However, perfluorocarbons, which are best suited for imaging – like perfluoro-15-crown-5 ether (PFCE) - tend to have extremely long biological retention. Here, we showed that the use of a multi-core PLGA nanoparticle entrapping PFCE allows for a 15-fold reduction of half-life in vivo compared to what is reported in literature. This unexpected rapid decrease in 19 F signal was observed in liver, spleen and within the infarcted region after myocardial infarction and was confirmed by whole body NMR spectroscopy. We demonstrate that the fast clearance is due to disassembly of the ~200 nm nanoparticle into ~30 nm domains that remain soluble and are cleared quickly. We show here that the nanoparticle ultrastructure has a direct impact on in vivo clearance of its cargo i.e. allowing fast release of PFCE, and therefore also bringing the possibility of multifunctional nanoparticle-based imaging to translational imaging, therapy and diagnostics. Graphical abstract: Image 1 Highlights: Perfluorocarbons (PFCs) are known to have long in vivo retention times. NP (nanoparticle) ultra-structure influences in vivo clearance properties. Clearance of PFCs in multi-core NPs is 15-times faster than single core NCs and NEs. PFC-containing multi-core NPs degrade into smaller domains with fast clearance. Multicore NPs clear faster from inflammatory foci such as myocardial infarctions.