Miniaturized Fluorimetric Method for Quantification of Zinc in Dry Dog Food. (4th September 2020)

• Record Type: Journal Article
• Authors: Martins, Rute C.; Pereira, Ana M.; Matos, Elisabete; Barreiros, Luisa; Fonseca, António J. M.; Cabrita, Ana R. J.; Segundo, Marcela A.
• Other Names: Trujillo Rodríguez María José Academic Editor.
• Is Part Of: Journal of analytical methods in chemistry. Volume 2020(2020)
• Publication Date: 2020-09-04
• Subjects: Chemistry, Analytic -- Periodicals; Chemistry, Analytic -- Technique -- Periodicals; 543.05
• DOI: 10.1155/2020/8821809
• Languages: English
• ISSNs: 2090-8865

Abstract

Abstract : Zinc is an essential trace element for animals in several biological processes, particularly in energy production, and it is acquired from food ingestion. In this context, a microplate-based fluorimetric assay was developed for simple, fast, and low-cost determination of zinc in pet food using 2, 2′-((4-(2, 7-difluoro-3, 6-dihydroxy-4a H -xanthen-9-yl)-3-methoxyphenyl)azanediyl)diacetic acid (FluoZin-1) as fluorescent probe. Several aspects were studied, namely, the stability of the fluorescent product over time, the FluoZin-1 concentration, and the pH of reaction media. The developed methodology provided a limit of detection of 1 μ g L −1 in sample acid digests, with a working range of 10 to 200 μ g L −1, corresponding to 100–2000 mg of Zn per kg of dry dog food samples. Intraday repeatability and interday repeatability were assessed, with relative standard deviation values < 3.4% (100 μ g L −1 ) and <11.7% (10 μ g L −1 ). Sample analysis indicated that the proposed fluorimetric assay provided results consistent with ICP-MS analysis. These results demonstrated that the developed assay can be used for rapid determination of zinc in dry dog food.