Functionalized hydrogel microparticles prepared by microfluidics and their interaction with tumour marker carbonic anhydrase IX. Issue 37 (1st September 2020)

• Record Type: Journal Article
• Authors: Pittermannová, A.; Ruberová, Z.; Lizoňová, D.; Hubatová-Vacková, A.; Kašpar, O.; Zadražil, A.; Král, V.; Pechar, M.; Pola, R.; Bibette, J.; Bremond, N.; Štěpánek, F.; Tokárová, V.
• Is Part Of: Soft matter. Volume 16:Issue 37(2020)
• Page Start: 8702
• Page End: 8709
• Publication Date: 2020-09-01
• Subjects: Soft condensed matter -- Periodicals; 530.413
• DOI: 10.1039/d0sm01018a
• Languages: English
• ISSNs: 1744-683X

Abstract

Abstract : Microfluidic fabrication of alginate particles with controlled size, followed by modifications to target tumour marker carbonic anhydrase IX. Abstract : Microfluidics allows precise control of the synthesis of microparticles for specific applications, where size and morphology play an important role. In this work, we have introduced microfluidic chip design with dedicated extraction and gelation sections allowing to prepare hydrogel particles in the size range of a red blood cell. The influence of the extractive channel size, alginate concentration and type of storage media on the final size of the prepared alginate microparticles has been discussed. The second part of the work is dedicated to the surface modification of prepared particles using chitosan, pHPMA and the monoclonal antibody molecule, IgG M75. The specific interaction of the antibody molecule with an antigen domain of carbonic anhydrase IX, the transmembrane tumour protein associated with several types of cancer, is demonstrated by fluorescence imaging and compared to an isotypic antibody molecule.