Aromatic carbohydrate amphiphile disrupts cancer spheroids and prevents relapse. Issue 37 (21st September 2020)

• Record Type: Journal Article
• Authors: Brito, Alexandra; Pereira, Patrícia M. R.; Reis, Rui L.; Ulijn, Rein V.; Lewis, Jason S.; Pires, Ricardo A.; Pashkuleva, Iva
• Is Part Of: Nanoscale. Volume 12:Issue 37(2020)
• Page Start: 19088
• Page End: 19092
• Publication Date: 2020-09-21
• Subjects: Nanoscience -- Periodicals; Nanotechnology -- Periodicals; 620.505
• DOI: 10.1039/d0nr05008c
• Languages: English
• ISSNs: 2040-3364

Abstract

Abstract : Phosphorylated carbohydrate amphiphile acts as a potent antimetabolite due to glycolysis inhibition and in situ formation of supramolecular net. Its efficiency is higher in spheroids as compared to the conventional 2D cultures. Abstract : Spheroids recapitulate the organization, heterogeneity and microenvironment of solid tumors. Herein, we targeted spatiotemporally the accelerated metabolism of proliferative cells located on the spheroid surface that ensure structure maintenance and/or growth. We demonstrate that phosphorylated carbohydrate amphiphile acts as a potent antimetabolite due to glycolysis inhibition and to in situ formation of supramolecular net around spheroid surface where alkaline phosphatase is overexpressed. The efficiency of the treatment is higher in spheroids as compared to the conventional 2D cultures because of the 2-fold higher expression of glucose transporter 1 (GLUT1). Moreover, treated spheroids do not undergo following relapse.