Reprocessing of Contaminated MGIT 960 Cultures to Improve Availability of Valid Results for Mycobacteria. (18th July 2020)

• Record Type: Journal Article
• Authors: Subramanyam, Balaji; Sivaramakrishnan, Gomathi; Sangamithrai, Devi; Ravi, Rajkumar; Thiruvengadam, Kannan; Vijayaragavan, Vaishnavi; Selvaraju, Ayswarya; Tripathy, Srikanth Prasad; Mondal, Rajesh
• Other Names: Callaway Todd R. Academic Editor.
• Is Part Of: International journal of microbiology. Volume 2020(2020)
• Publication Date: 2020-07-18
• Subjects: Microbiology -- Periodicals; Microbiology; Microbiology -- Periodicals; Electronic journals; Periodicals; 579
• DOI: 10.1155/2020/1721020
• Languages: English
• ISSNs: 1687-918X

Abstract

Abstract : Optimal recovery of mycobacteria from the contaminated liquid culture is a challenge. While alternative methods have been suggested to reduce the rate of contamination in the BACTEC MGIT 960 system, reprocessing the contaminated liquid culture improves recovery of Mycobacterium tuberculosis . Among 793 MGIT cultures raised from as many sputum specimens after primary decontamination by the standard NaLC-NaOH method, valid results were available for 687 (86.6%) as 106 (13.4%) were contaminated. Reprocessing and reculturing of the contaminated cultures increased valid results to 739 (93.2%) and reduced the contamination rate to 6.8%. Both values were statistically significant. Recovery of the Mycobacterium tuberculosis complex increased from 45.6% to 48.4%. Valid negative results were available for an additional 3.4%. The method may be adopted to reduce the rate of contamination and to improve the valid culture results for mycobacteria.