Continuous Assembly of β-Roll Structures Is Implicated in the Type I-Dependent Secretion of Large Repeat-in-Toxins (RTX) Proteins. Issue 20 (18th September 2020)

• Record Type: Journal Article
• Authors: Motlova, Lucia; Klimova, Nela; Fiser, Radovan; Sebo, Peter; Bumba, Ladislav
• Is Part Of: Journal of molecular biology. Volume 432:Issue 20(2020)
• Page Start: 5696
• Page End: 5710
• Publication Date: 2020-09-18
• Subjects: adenylate cyclase toxins -- type I secretion system -- RTX proteins -- Bordetella pertussis; AC adenylate cyclase -- RTX Repeats-in-Toxins -- T1SS type I secretion system -- CyaA adenylate cyclase toxin -- CR3 complement receptor 3 -- CD circular dichroism -- SAXS small-angle X-ray scattering -- GST glutathione S-transferase; Molecular biology -- Periodicals; Biology -- Periodicals; Biochemistry -- Periodicals; Bacteriology -- Periodicals; Molecular Biology -- Periodicals; Biochemistry -- Periodicals; Biologie moléculaire -- Périodiques; Biologie -- Périodiques; Biochimie -- Périodiques; Moleculaire biologie; Biochemistry; Biology; Molecular biology; Periodicals; 572.805
• DOI: 10.1016/j.jmb.2020.08.020
• Languages: English
• ISSNs: 0022-2836

Abstract

Abstract: Repeats-in-Toxin (RTX) proteins of Gram-negative bacteria are excreted through the type I secretion system (T1SS) that recognizes non-cleavable C-terminal secretion signals. These are preceded by arrays of glycine and aspartate-rich nonapeptide repeats grouped by four to eight β strands into blocks that fold into calcium-binding parallel β-roll structures. The β-rolls are interspersed by linkers of variable length and sequence and the organization of multiple RTX repeat blocks within large RTX domains remains unknown. Here we examined the structure and function of the RTX domain of Bordetella pertussis adenylate cyclase toxin (CyaA) that is composed of five β-roll RTX blocks. We show that the non-folded RTX repeats maintain the stability of the CyaA polypeptide in the Ca 2+ -depleted bacterial cytosol and thereby enable its efficient translocation through the T1SS apparatus. The efficacy of secretion of truncated CyaA constructs was dictated by the number of retained RTX repeat blocks and depended on the presence of extracellular Ca 2+ ions. We further describe the crystal structure of the RTX blocks IV–V of CyaA (CyaA1372 – 1681 ) that consists of a contiguous assembly of two β-rolls that differs substantially from the arrangement of the RTX blocks observed in RTX lipases or other RTX proteins. These results provide a novel structural insight into the architecture of the RTX domains of large RTX proteins and support the "push-ratchet" mechanism of the T1SS-mediated secretion of very large RTX proteins. Graphical abstract: Unlabelled Image Highlights: Calcium-binding RTX repeats operate in a dual structure–function regime. The non-folded RTX repeats maintain the stability of the T1SS substrates. The number of RTX repeats dictates the secretion efficacy of large RTX substrates. The RTX domain of the CyaA toxin forms an extended parallel β-roll structure.