N2O production using native nos-deficient denitrifying bacterial strains screened by a genome mining approach. (September 2020)

• Record Type: Journal Article
• Authors: Oshiki, Mamoru; Ishimaru, Miho; Hatamoto, Masashi; Yamaguchi, Takashi; Araki, Nobuo; Okabe, Satoshi
• Is Part Of: Bioresource technology reports. Volume 11(2020)
• Publication Date: 2020-09
• Subjects: Nitrogen removal -- Nitrous oxide (N2O) -- N2O reductase (Nos) -- nos-deficient denitrifying bacteria -- Genome mining; Biomass energy -- Periodicals; Biotransformation (Metabolism) -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Waste products as fuel -- Periodicals; Waste products as fuel; Organic wastes; Factory and trade waste; Biotransformation (Metabolism); Biomass energy; Agricultural wastes; Periodicals; Electronic journals; 662.88
• DOI: 10.1016/j.biteb.2020.100529
• Languages: English
• ISSNs: 2589-014X

Abstract

Abstract: Although N2 O is a notorious greenhouse and ozone-depleting gas, N2 O is a strong oxidant and can be an energy source by combusting with methane gas. The present study screened N2 O-producing denitrifying bacteria by genome mining approach, and the activity and efficiency of NO2 − reduction to N2 O were examined. Presence and absence of the genes encoding nitrite reductase ( nir ), nitric oxide reductase ( nor ), and nitrous oxide reductase ( nos ) on the 2750 prokaryotic genomes were examined to screen nos -deficient denitrifying bacteria capable of NO2 − reduction to N2 O. The 131 nos -deficient denitrifying bacterial genomes were screened, and Cupriavidus necator H16 and Propionibacterium freudenreichii showed high activity and efficiency of 15 NO2 − reduction to 15 N2 O. Nitrogen removal performance and N2 O conversion efficiencies were further investigated in a membrane bioreactor, and C. necator H16 showed 0.18 kg-N m −3 d −1 of NO2 − removal rate with 75–95% of N2 O conversion efficiencies. Graphical abstract: Unlabelled Image Highlights: N2 O is a strong oxidant and can be an energy source. Genome mining is a systematic approach for screening nos -deficient bacteria. 131 nos -deficient denitrifying bacterial genomes were screened. Cupriavidus necator H16 showed high N2 O production activity. Abiotic NO2 − reduction occurred in Propionibacterium freudenreichii culture.