ATG7 is essential for secretion of iron from ameloblasts and normal growth of murine incisors during aging. Issue 10 (2nd October 2020)

• Record Type: Journal Article
• Authors: Sukseree, Supawadee; Schwarze, Uwe Yacine; Gruber, Reinhard; Gruber, Florian; Quiles Del Rey, Maria; Mancias, Joseph D.; Bartlett, John D.; Tschachler, Erwin; Eckhart, Leopold
• Is Part Of: Autophagy. Volume 16:Issue 10(2020)
• Page Start: 1851
• Page End: 1857
• Publication Date: 2020-10-02
• Subjects: Aging -- ameloblast -- ATG7 -- autophagy -- epithelium -- ferritin -- hyperplasia -- iron -- secretion -- tooth; Autophagic vacuoles -- Periodicals; Apoptosis -- Periodicals; Cell death -- Periodicals; Lysosomes -- Periodicals; Degeneration (Pathology) -- Periodicals; Autophagy -- Periodicals; Cell Death -- Periodicals; Lysosomes -- Periodicals; Periodicals; 571.936
• DOI: 10.1080/15548627.2019.1709764
• Languages: English
• ISSNs: 1554-8627

Abstract

ABSTRACT: The incisors of rodents comprise an iron-rich enamel and grow throughout adult life, making them unique models of iron metabolism and tissue homeostasis during aging. Here, we deleted Atg7 (autophagy related 7) in murine ameloblasts, i.e. the epithelial cells that produce enamel. The absence of ATG7 blocked the transport of iron from ameloblasts into the maturing enamel, leading to a white instead of yellow surface of maxillary incisors. In aging mice, lack of ATG7 was associated with the growth of ectopic incisors inside severely deformed primordial incisors. These results suggest that 2 characteristic features of rodent incisors, i.e. deposition of iron on the enamel surface and stable growth during aging, depend on autophagic activity in ameloblasts. Abbreviations : ATG5: autophagy related 5; ATG7: autophagy related 7; CMV: cytomegalovirus; Cre: Cre recombinase; CT: computed tomography; FTH1: ferritin heavy polypeptide 1; GFP: green fluorescent protein; KRT5: keratin 5; KRT14: keratin 14; LGALS3: lectin, galactose binding, soluble 3; MAP1LC3/LC3: microtubule-associated protein 1 light chain 3; NCOA4: nuclear receptor coactivator 4; NRF2: nuclear factor, erythroid 2 like 2; SQSTM1: sequestosome 1.