Enzyme-free electrochemical sensor for the determination of hydrogen peroxide secreted from MCF-7 breast cancer cells using calcined indium metal-organic frameworks as efficient catalysts. (1st November 2020)

• Record Type: Journal Article
• Authors: Wei, Ping; Sun, Duanping; Niu, Yuanyuan; Lu, Xiange; Zhai, Haiyun
• Is Part Of: Electrochimica acta. Volume 359(2020)
• Publication Date: 2020-11-01
• Subjects: Electrochemistry -- Metal-organic framework -- Electrodeposition -- Hydrogen peroxide -- MCF-7 breast cancer cells; Electrochemistry -- Periodicals; Electrochemistry, Industrial -- Periodicals; 541.37
• DOI: 10.1016/j.electacta.2020.136962
• Languages: English
• ISSNs: 0013-4686

Abstract

Highlights: Calcined MIL-68 were used to real-time quantification of H2 O2 from MCF-7 cancer cells. Calcined indium metal-organic frameworks materials were synthesized by changing the time. Pt/cMIL-68/MoS2/GCE exhibited the best electrocatalytic performance for H2 O2 reduction. The sensor showed high selectivity and sensitivity with detection limit of 6.26 nM in situ. Abstract: As a major member of the reactive oxygen species, hydrogen peroxide (H2 O2 ) is a vital endogenous marker for oxidative stress analysis. Herein, we report an enzyme-free sensor for the electrochemical detection of H2 O2 based on the electrodeposition of platinum (Pt) nanoparticles (NPs) on calcined metal-organic framework (MOF) MIL-68-NH2 (In) and molybdenum disulfide (MoS2 ) nanosheets. Firstly, the glassy carbon electrode (GCE) was coated by two-dimensional MoS2 nanosheets, which can load more MOF materials. Interestingly, we have found that calcined MIL-68 (cMIL-68) displays the better catalytic activity than pristine MIL-68 because of the larger surface area and better electrical conductivity. And the cMIL-68 exhibits the best electrocatalytic property for H2 O2 after 3 h calcination. To enhance the detection sensitivity, Pt NPs was electrodeposited on the cMIL-68/MoS2 /GCE and can offer better electrical conductivity and stability. Therefore, the incorporation of three materials provides the superior selective and sensitive detection of H2 O2 . Under the optimal conditions, a wide linear range was shown in 10 nM to 18.3 mM and a low detection limit was found to be 6.26 nM. Furthermore, Pt/cMIL-68/MoS2 /GCE was applied for real-time quantification of H2 O2 released from MCF-7 breast cancer cells. These results suggest that the calcined MOF materials have the great potential for improving the detection sensitivity of sensor in monitoring physiological processes. Graphical abstract: Image, graphical abstract