Circulating miRNA-23b and miRNA-143 Are Potential Biomarkers for In-Stent Restenosis. (17th September 2020)

• Record Type: Journal Article
• Authors: Saavedra, Nicolás; Rojas, Gabriel; Herrera, Jesús; Rebolledo, Camilo; Ruedlinger, Jenny; Bustos, Luis; Bobadilla, Braulio; Pérez, Luis; Saavedra, Kathleen; Zambrano, Tomás; Lanas, Fernando; Salazar, Luis A.
• Other Names: Ielapi Nicola Academic Editor.
• Is Part Of: BioMed research international. Volume 2020(2020)
• Publication Date: 2020-09-17
• Subjects: Medicine -- Periodicals; Biology -- Periodicals; Biotechnology -- Periodicals; Life sciences -- Periodicals; 610.5
• DOI: 10.1155/2020/2509039
• Languages: English
• ISSNs: 2314-6133

Abstract

Abstract : In-stent restenosis (ISR) is one of the main complications in patients undergoing percutaneous coronary angioplasty, and microRNAs participate in the contractile-to-synthetic phenotypic switch of vascular smooth muscle cells, a hallmark of restenosis development. MicroRNAs (miRNAs) can be released into circulation from injured tissues, enticing a potential role as noninvasive biomarkers. We aimed to evaluate circulating levels of miRNA-23b, miRNA-143, and miRNA-145 as diagnostic markers of ISR. 142 patients with coronary artery disease undergoing successful angioplasty and a follow-up angiography were included. Subjects were classified according to the degree of obstruction at the angioplasty site into cases (≥50%) or controls (<50%). Total RNA was isolated from plasma to quantify circulating miRNAs levels, and the ROC curves were constructed. Among circulating miRNAs assessed, miRNA-23b and miRNA-143 were significantly lower in cases (miRNA-23b: 18.4 x 10 − 5 and miRNA-143: 13.7 x 10 − 5 ) than controls (miRNA-23b: 5.2 x 10 − 5, p < 0.0001 ; miRNA-143: 4.0 x 10 − 5, p < 0.0001 ). Plasma levels of miRNA-145 showed no significant differences. The analysis of the ROC curves showed an area under the curve for miRNA-23b of 0.71 (95% CI: 0.62-0.80, p < 0.0001 ) and 0.69 for miRNA-143 (95% CI: 0.60-0.78; p < 0.0001 ). Our data suggest that plasma levels of miRNA-23b and miRNA-143 could be useful as noninvasive biomarkers of ISR.