Detection of Extended-Spectrum β-Lactamases (ESBL) Producing Enterobacteriaceae from Fish Trapped in the Lagoon Area of Bizerte, Tunisia. (10th June 2020)

• Record Type: Journal Article
• Authors: Hassen, Bilel; Jouini, Ahlem; Elbour, Monia; Hamrouni, Safa; Maaroufi, Abderrazek
• Other Names: Schneider György Academic Editor.
• Is Part Of: BioMed research international. Volume 2020(2020)
• Publication Date: 2020-06-10
• Subjects: Medicine -- Periodicals; Biology -- Periodicals; Biotechnology -- Periodicals; Life sciences -- Periodicals; 610.5
• DOI: 10.1155/2020/7132812
• Languages: English
• ISSNs: 2314-6133

Abstract

Abstract : Extended-spectrum β -lactamase and their molecular mechanism in Enterobacteriaceae were analyzed in 126 fish samples of 9 various wild species, living in the lagoon of Bizerte in Tunisia. Fifty-nine (59) Gram-negative strains were isolated and identified as Escherichia coli (n = 24 ), Klebsiella pneumonia (n = 21 ), Citrobacter freundii (n = 8 ), and Shigella boydii (n = 6 ). Forty-seven ESBL producers were identified using the synergic test. β -Lactamase genes detected were bla CTX-M-1 ( E. coli /15; K. pneumonia /8; C. freundii /1; Sh. boydii /1), bla CTX-M-1 + bla OXA-1 ( E. coli /4; K. pneumonia /3), bla CTX-M-1 + bla TEM-1-a ( K. pneumonia /2), bla CTX-M-15 + bla TEM-1-a ( K. pneumonia /1; Sh. boydii /1), bla CTX-M-15 + bla OXA-1 ( K. pneumonia /1), bla CTX-M-15 ( E. coli /3; K. pneumonia /1; Sh. boydii /3), and bla CTX-M-9 ( C. freundii /3). Most strains (84.7%) showed a multiresistant phenotype. qnrA and qnrB genes were identified in six E. coli and in ten E. coli +one K. pneumonia isolates, respectively. The resistance to tetracycline and sulfonamide was conferred by the tet and sul genes. Characterization of phylogenic groups in E. coli isolates revealed phylogroups D (n = 20 strains), B2 (n = 2 ), and A (n = 2 ). The studied virulence factor showed prevalence of fimA genes in 9 E. coli isolates (37.5%). Similarly, no strain revealed the three other virulence factors tested ( eae, aer, and cnf1 ). Our findings confirmed that the lagoons of Bizerte may be a reservoir of multidrug resistance/ESBL-producing Enterobacteriaceae . This could lead to indisputable impacts on human and animal health, through the food chain.