NIR-II window tracking of hyperglycemia induced intracerebral hemorrhage in cerebral cavernous malformation deficient mice. (21st August 2020)

• Record Type: Journal Article
• Authors: Parchur, Abdul K.; Fang, Zhi; Jagtap, Jaidip M.; Sharma, Gayatri; Hansen, Christopher; Shafiee, Shayan; Hu, Wenquan; Miao, Qing R.; Joshi, Amit
• Is Part Of: Biomaterials science. Volume 8:Number 18(2020)
• Page Start: 5133
• Page End: 5144
• Publication Date: 2020-08-21
• Subjects: Biomedical materials -- Periodicals; 610.28
• DOI: 10.1039/d0bm00873g
• Languages: English
• ISSNs: 2047-4830

Abstract

Abstract : Left panel: Pseudocolor map of 3 principle components from NIR-II kinetic imaging, Right panel (top to bottom): In vivo Ag2 S QD NIR-II fluorescence, ex vivo iodine micro-CT, FITC dextran perfusion, and H&E staining in control vs CCM1+/− mice brain. Abstract : Second near infrared (NIR-II) window fluorescence imaging between 1000 and 1700 nm with reduced scattering and autofluorescence and deep tissue light penetration allows early and non-invasive determination of vascular pathologies. Here, we demonstrate in vivo NIR-II imaging techniques for tracking hyperglycaemia-induced Intracerebral Hemorrhage (ICH) and Blood Brain Barrier (BBB) hyperpermeability in Cerebral Cavernous Malformation (CCM) deficient mice (CCM1+/−). We synthesised PEGylated Ag2 S quantum dots (QDs) with a bright fluorescent emission peak centred at 1135 nm under an 808 nm NIR light for dynamic imaging of cerebral vasculature in mice and determined the development of ICH and BBB impairment in hyperglycaemic CCM1+/− mice. In vivo optical imaging was conducted with micro-CT (including k -mean cluster analysis) as well as in vivo permeability assays using FITC-dextran perfusion and IgG staining, respectively. The increased BBB permeability in CCM1+/− mice was further demonstrated to be associated with a high-glucose-caused decrease of CCM1 expressions. This study validates that deep-penetrating NIR-II QDs can be used for the tracking of ICH and BBB hyperpermeability in transgenic mice models of cerebral vascular anomalies.