A selective purine-based fluorescent chemosensor for the "naked-eye" detection of zinc ions (Zn2+): applications in live cell imaging and test strips. (27th August 2020)

• Record Type: Journal Article
• Authors: Xu, Haiyan; Chen, Wei; Zhang, Weixia; Ju, Lixin; Lu, Hongfei
• Is Part Of: New journal of chemistry. Volume 44:Number 35(2020)
• Page Start: 15195
• Page End: 15201
• Publication Date: 2020-08-27
• Subjects: Chemistry -- Periodicals; Chimie -- Périodiques; 540
• DOI: 10.1039/d0nj02687e
• Languages: English
• ISSNs: 1144-0546

Abstract

Abstract : In this work, a novel purine based probe PTAHN was successfully designed and synthesized. PTAHN displayed high selectivity towards Zn 2+ via turn-on fluorescence. What's more, PTAHN can be proficiently employed for imaging Zn 2+ in living cells. Abstract : A new "turn-on" fluorescent probe (PTAHN ) based on a biological purine derivative for detecting Zn 2+ was designed, synthesized and characterized. The PTAHN exhibited remarkable selectivity and rapid response toward Zn 2+, as well as obvious fluorescence enhancement upon combining with Zn 2+ in a HEPES buffer system at pH 7.4. The limit of detection (LOD) for Zn 2+ (61.6 nM) is much lower than the WHO standards (76.0 μM). Meanwhile, an obvious color change can be visually observed by the naked eye between PTAHN and PTAHN –Zn 2+ . What's more, the sensing mechanism of PTAHN toward Zn 2+ was supported by the Job's plot, 1 H NMR and density functional theory (DFT) studies. Interestingly, the novel probe is cell-permeable and can be successfully used for the detection of intracellular zinc ions in living HepG-2 cells.