Uncultivated stromal vascular fraction is equivalent to adipose‐derived stem and stromal cells on porous polyurethrane scaffolds forming adipose tissue in vivo. (15th February 2018)

• Record Type: Journal Article
• Authors: Griessl, Michael; Buchberger, Anna‐Maria; Regn, Sybille; Kreutzer, Kilian; Storck, Katharina
• Is Part Of: Laryngoscope. Volume 128:Number 6(2018)
• Page Start: E206
• Page End: E213
• Publication Date: 2018-02-15
• Subjects: Adipose‐derived stem and stromal cells -- tissue engineering -- fat transfer -- vascularization -- adipogenesis -- angiogenesis -- stromal vascular fraction -- stromal vascular fraction -- arteriovenous flow‐through; Otolaryngology -- Periodicals; 617.51005
• DOI: 10.1002/lary.27124
• Languages: English
• ISSNs: 0023-852X

Abstract

Abstract : Objectives/Hypothesis: To find an alternative approach to contemporary techniques in tissue augmentation and reconstruction, tissue engineering strategies aim to involve adipose‐derived stem and stromal cells (ASCs) harboring a strong differentiation potential into various tissue types such as bone, cartilage, and fat. Study Design: Animal research. Methods: The stromal vascular fraction (SVF) was used directly as a cell source to provide a potential alternative to contemporary ASC‐based adipose tissue engineering. Seeded in TissuCol fibrin, we applied ASCs or SVF cells to porous, degradable polyurethane (PU) scaffolds. Results: We successfully demonstrated the in vivo generation of volume‐stable, well‐vascularized PU‐based constructs containing host‐derived mature fat pads. Seeded human stem cells served as modulators of host‐cell migration rather than differentiating themselves. We further demonstrated that preliminary culture of SVF cells was not necessary. Conclusions: Our results bring adipose tissue engineering, together with automated processing devices, closer to clinical applicability. The time‐consuming and cost‐intensive culture and induction of the ASCs is not necessary. Level of Evidence: NA. Laryngoscope, 128:E206–E213, 2018