Mesoscale diffusion magnetic resonance imaging of the ex vivo human hippocampus. Issue 15 (4th July 2020)

• Record Type: Journal Article
• Authors: Ly, Maria; Foley, Lesley; Manivannan, Ashwinee; Hitchens, T. Kevin; Richardson, R. Mark; Modo, Michel
• Is Part Of: Human brain mapping. Volume 41:Issue 15(2020)
• Page Start: 4200
• Page End: 4218
• Publication Date: 2020-07-04
• Subjects: connectome -- diffusion tensor imaging -- epilepsy -- ex vivo -- hippocampus -- mesoscale -- tractography; Brain mapping -- Periodicals; 611.81
• DOI: 10.1002/hbm.25119
• Languages: English
• ISSNs: 1065-9471

Abstract

Abstract: Mesoscale diffusion magnetic resonance imaging (MRI) endeavors to bridge the gap between macroscopic white matter tractography and microscopic studies investigating the cytoarchitecture of human brain tissue. To ensure a robust measurement of diffusion at the mesoscale, acquisition parameters were arrayed to investigate their effects on scalar indices (mean, radial, axial diffusivity, and fractional anisotropy) and streamlines (i.e., graphical representation of axonal tracts) in hippocampal layers. A mesoscale resolution afforded segementation of the pyramidal cell layer (CA1‐4), the dentate gyrus, as well as stratum moleculare, radiatum, and oriens. Using ex vivo samples, surgically excised from patients with intractable epilepsy ( n = 3), we found that shorter diffusion times (23.7 ms) with a b‐value of 4, 000 s/mm 2 were advantageous at the mesoscale, providing a compromise between mean diffusivity and fractional anisotropy measurements. Spatial resolution and sample orientation exerted a major effect on tractography, whereas the number of diffusion gradient encoding directions minimally affected scalar indices and streamline density. A sample temperature of 15°C provided a compromise between increasing signal‐to‐noise ratio and increasing the diffusion properties of the tissue. Optimization of the acquisition afforded a system's view of intra‐ and extra‐hippocampal connections. Tractography reflected histological boundaries of hippocampal layers. Individual layer connectivity was visualized, as well as streamlines emanating from individual sub‐fields. The perforant path, subiculum and angular bundle demonstrated extra‐hippocampal connections. Histology of the samples confirmed individual cell layers corresponding to ROIs defined on MR images. We anticipate that this ex vivo mesoscale imaging will yield novel insights into human hippocampal connectivity. Abstract : Mesoscale diffusion magnetic resonance imaging (MRI) endeavors to bridge the gap between macroscopic white matter tractography and microscopic studies investigating the cytoarchitecture of human brain tissue. We here optimized the mesoscale acquisition of diffusion MRI on the human hippocampus to resolve intra‐hippocampal connections which are relevant to neurological conditions, such as epilepsy and Alzheimer's disease.