Reversible spatial and temporal control of lipid signaling. Issue 73 (28th August 2020)

• Record Type: Journal Article
• Authors: Gaur, Pankaj; Galkin, Maksym; Hauke, Sebastian; Redkin, Ruslan; Barnes, Carolyn; Shvadchak, Volodymyr V.; Yushchenko, Dmytro A.
• Is Part Of: Chemical communications. Volume 56:Issue 73(2020)
• Page Start: 10646
• Page End: 10649
• Publication Date: 2020-08-28
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d0cc04146g
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Fluorescent cages for selective delivery of photoswitchable lipids to specific cell membranes for high-precision studies of lipid signaling. Abstract : Herein, we introduce versatile molecular tools that enable specific delivery and visualization of photoswitchable lipids at cellular membranes, namely at the plasma membrane and internal membranes. These molecules were prepared by tethering ortho -nitrobenzyl-based fluorescent cages with a signaling lipid bearing an azobenzene photoswitch. They permit two sequential photocontrolled reactions, which are uncaging of a lipid analogue and then its repeated activation and deactivation. We used these molecules to activate GPR40 receptor transiently expressed in HeLa cells and demonstrated downstream modulation of intracellular Ca 2+ levels.