A set of rhamnosylation-specific antibodies enables detection of novel protein glycosylations in bacteria. Issue 35 (2nd September 2020)

• Record Type: Journal Article
• Authors: Gast, Daniel; Koller, Franziska; Krafczyk, Ralph; Bauer, Lukas; Wunder, Swetlana; Lassak, Jürgen; Hoffmann-Röder, Anja
• Is Part Of: Organic & biomolecular chemistry. Volume 18:Issue 35(2020)
• Page Start: 6823
• Page End: 6828
• Publication Date: 2020-09-02
• Subjects: Chemistry, Organic -- Periodicals; Bioorganic chemistry -- Periodicals; Chemistry, Physical organic -- Periodicals; 547
• DOI: 10.1039/d0ob01289k
• Languages: English
• ISSNs: 1477-0520

Abstract

Abstract : Chemical curiosity or not? Specific antibodies to study the rhamnoproteome in bacteria. Abstract : Despite its potential importance for bacterial virulence, protein rhamnosylation has not yet been sufficiently studied. Specific anti -Ser Rha, anti -Thr Rha and anti -Asn Rha antibodies allowed the identification of previously unknown monorhamnosylated proteins in cytosol and membrane fractions of bacterial cell lysates. Mapping of the complete rhamnoproteome in pathogens should facilitate development of targeted therapies against bacterial infections.