Quercitrin, the Main Compound in Wikstroemia indica, Mitigates Skin Lesions in a Mouse Model of 2, 4-Dinitrochlorobenzene-Induced Contact Hypersensitivity. (9th July 2020)

• Record Type: Journal Article
• Authors: Jegal, Jonghwan; Park, No-June; Lee, So-Yeon; Jo, Beom-Geun; Bong, Sim-Kyu; Kim, Su-Nam; Yang, Min Hye
• Other Names: Shi Xiangguo Guest Editor.
• Is Part Of: Evidence-based complementary and alternative medicine. Volume 2020(2020)
• Publication Date: 2020-07-09
• Subjects: Alternative medicine -- Periodicals; 615.505
• DOI: 10.1155/2020/4307161
• Languages: English
• ISSNs: 1741-427X

Abstract

Abstract : Hapten-induced contact hypersensitivity (CHS) is widely utilized to induce immune activation in animal models of allergic contact dermatitis. Our previous findings suggested that the 95% EtOH extract of Wikstroemia indica (L.) C. A. Mey. has antiallergic and anti-inflammatory effects in DNCB-treated CHS SKH-1 hairless mice. The aim of this study was to evaluate the protective effects of compounds isolated from the EtOAc fraction of W. indica in RBL-2H3 cells and 2, 4-dinitrochlorobenzene- (DNCB-) induced CHS mice. Of eight compounds in W. indica, that is, umbelliferone, daphnoretin, wikstrocoumarin, (+)-syringaresinol, tricin, (+)-lariciresinol, erythro -guaiacylglycerol-β -coniferyl ether, and quercitrin, quercitrin exhibited the most antiallergic activity against antigen-induced β -hexosaminidase release and IL-4 mRNA expression, which are markers of degranulation in RBL-2H3 cells. After a 7-sensitizing period, 14 days of DNCB treatment with or without topical pimecrolimus (1%) or quercitrin (0.5%) treatment, quercitrin was found to suppress DNCB-induced increases in serum IL-4 and IgE concentrations and transepidermal water loss. These results indicate that quercitrin has therapeutic potential for treatment of allergies and allergy-related contact dermatitis.