Optimization Extraction of Shikonin Using Ultrasound-Assisted Response Surface Methodology and Antibacterial Studies. (29th July 2020)

• Record Type: Journal Article
• Authors: Huang, Xiang-Yue; Fu, Hua-Lin; Tang, Hua-Qiao; Yin, Zhong-Qiong; Zhang, Wei; Shu, Gang; Yin, Li-Zi; Zhao, Ling; Yan, Xiao-Rong; Lin, Ju-Chun
• Other Names: Mukudai Yoshiki Academic Editor.
• Is Part Of: Evidence-based complementary and alternative medicine. Volume 2020(2020)
• Publication Date: 2020-07-29
• Subjects: Alternative medicine -- Periodicals; 615.505
• DOI: 10.1155/2020/1208617
• Languages: English
• ISSNs: 1741-427X

Abstract

Abstract : The objectives of this study were to develop and optimize ultrasound-assisted extraction (UAE) for shikonin from Arnebia euchroma using response surface methodology (RSM) and to evaluate the antimicrobial activity of shikonin. The maximum yield of shikonin was 1.26% under the optimal extraction conditions (ultrasound power, 93 W; time, 87 min; temperature, 39°C; and liquid-solid ratio, 11 : 1). Shikonin showed inhibitory activity against standard strains and clinical isolates to varying extents (MICs ranging from 128 to 1024 μ g/mL, MBCs ranging from 256 to 2048 μ g/mL), and it was more effective for Gram-positive bacteria as indicated by lower MIC and MBC values. Time-kill curves revealed that antibacterial activity of shikonin exhibited a dose-response relationship. In summary, via this study, we identified ultrasound-assisted RSM as the optimal extraction method for shikonin, which is a potential material for the treatment of bacterial infections.