Fatty Acid Composition, Phospholipid Molecules, and Bioactivities of Lipids of the Mud Crab Scylla paramamosain. (19th March 2020)

• Record Type: Journal Article
• Authors: Nguyen, Thi Phuong Lan; Nguyen, Van Tuyen Anh; Do, Thi Thanh Trung; Nguyen Quang, Trung; Pham, Quoc Long; Le, Tat Thanh
• Other Names: Caulfield Thomas R. Academic Editor.
• Is Part Of: Journal of chemistry. Volume 2020(2020)
• Publication Date: 2020-03-19
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1155/2020/8651453
• Languages: English
• ISSNs: 2090-9063

Abstract

Abstract : The mud crab increases the yield of farming in Southeast Asian countries. Scylla paramamosain, one of four mud crab species belonging to the Scylla genus, is a rich nutrient source during its soft-shell moulting period. In this study, we analysed the total lipid content, fatty acid components, and phospholipid molecular species of the S . paramamosain mud crab. The total lipid content was 1.62 ± 0.08%, which is similar to that of S . serrata previously reported . Twenty-one fatty acids were identified in S . paramamosain . The composition and molecular forms of the phospholipids were identified using high-performance liquid chromatography-high-resolution mass spectrometry. Fifty-four different molecules belonging to six types of phospholipids were identified. Notably, phospholipids were made of fatty acids with C16 : 0; C18 : 0; C20 : 4; C20 : 5; and C22 : 6 main components. The anti-inflammatory and cytotoxic effects of crab lipids and phospholipids were investigated for the first time. The anti-inflammatory activity of the total and polar lipids had IC50 values of 71.5 and 68.6 μ g/mL, respectively. The crab polar lipid fraction, which contained phospholipids, also presented high cytotoxic activity toward five cancer cell lines with IC50 values ranging from 85.4 to 95.8 μ g/mL.