Rose Bengal-Mediated Photodynamic Antimicrobial Treatment of Acanthamoeba Keratitis. (2nd October 2020)

• Record Type: Journal Article
• Authors: Atalay, Hatice Tuba; Uysal, Betül Seher; Sarzhanov, Fakhriddin; Usluca, Selma; Yeşilırmak, Nilüfer; Özmen, Mehmet Cüneyt; Erganiş, Sidre; Tefon, Atike Burçin; Dogruman-Al, Funda; Bilgihan, Kamil
• Is Part Of: Current eye research. Volume 45:Number 10(2020)
• Page Start: 1205
• Page End: 1210
• Publication Date: 2020-10-02
• Subjects: Rose Bengal (RB) -- 0.1% and 0.2% RB -- RB-mediated photodynamic antimicrobial therapy (RB-PDAT) -- Acanthamoeba castellanii keratitis; Ophthalmology -- Periodicals; Eye -- Diseases -- Periodicals; Ophthalmology -- Periodicals; 573.88
• DOI: 10.1080/02713683.2020.1731830
• Languages: English
• ISSNs: 0271-3683

Abstract

ABSTRACT: Purpose : To evaluate the in vivo efficacy of rose bengal (RB)-mediated photodynamic antimicrobial therapy (PDAT) for treatment of Acanthamoeba castellanii keratitis (AK). Materials and Methods : An animal (rabbit) AK model was successfully achieved via intrastromal inoculation of a suspension of A. castellanii cells and trophozoites. Prior to RB-PDAT (pre-treatment, day-5), the severity of the induced corneal infection was graded numerically for epithelial defects, stromal edema, neovascularity, and stromal opacity/infiltration. The right eyes of rabbits (n = 18) were divided equally into three groups (n = 6/group): control (no treatment); 0.1% RB+518 nm irradiation (5.4 J/cm 2 ); and 0.2% RB+518 nm irradiation (5.4 J/cm 2 ). On post-treatment day-5, animals were euthanized, after which corneal buttons were excised and submitted for real-time polymerase chain reaction (RT-PCR) analysis. Results : Post-treatment clinical scores of the 0.1 and 0.2% RB groups indicated significant improvement compared to control group scores (pre-treatment clinical scores; 5.17 ± 0.98, 7.50 ± 0.62, and 6.17 ± 0.70 and post-treatment clinical scores; 4.50 ± 0.56, ( p = .043), 3.50 ± 0.99 ( p = .039), 6.83 ± 1.66 ( p = .34), respectively). RT-PCR analysis revealed that the mean cycle threshold (Ct) values were significantly higher in treated-group corneas compared to control-group corneas, with no significant differences between treated-groups (Mean Ct values; 34.33, 34.5, and 29.67 for 0.1 and 0.2% RB, and control groups). There was a statistically significant negative correlation between post-treatment clinical scores and Ct values ( r = −0.474, p -value 0.047). Conclusions : Our results demonstrate that RB-PDAT is effective in decreasing the parasitic load and clinical severity of AK.