Th9 Cells in Peripheral Blood Increased in Patients with Immune-Related Pancytopenia. (5th May 2020)

• Record Type: Journal Article
• Authors: Shao, Qing; Wang, Yangyang; Liu, Zhaoyun; Liu, Hui; Wang, Yihao; Zhao, Yang; Li, Lijuan; Fu, Rong
• Other Names: Milito Cinzia Academic Editor.
• Is Part Of: Journal of immunology research. Volume 2020(2020)
• Publication Date: 2020-05-05
• Subjects: Immunology -- Periodicals; Immunology -- Research -- Periodicals; 616.07905
• DOI: 10.1155/2020/6503539
• Languages: English
• ISSNs: 2314-8861

Abstract

Abstract : Background . Immune-related pancytopenia (IRP) is a kind of autoimmune disease mediated by autoantibodies in bone marrow. T helper 9 (Th9) cell is a new subset of T cell discovered recently, which mainly expresses cytokine interleukin-9 (IL-9) to exert immune function. Th9 cells are associated with a variety of inflammatory diseases, but the role of Th9 cells in IRP remains unclear. Methods . Fifty patients with IRP and 20 healthy controls were enrolled. The percentage of Th9 cells was detected by flow cytometry (FCM) and ELISA. CD4 + lymphocytes were sorted by magnetic beads, and the mRNA expression levels of Th9 cells related transcription factors PU.1 and BATF were detected by RT-PCR. Results . The percentage of Th9 cells in CD3 + CD4 + cells was 2.73 ± 1.96 % in the untreated group, which was significantly higher than those in the remission group (1.21 ± 0.86 % ) (p < 0.01 ) and the control group (0.68 ± 0.40 % ) (p < 0.001 ). And that in the remission group was significantly higher than that in the control group (p < 0.05 ). The level of IL-9 in the untreated group was 183.91 ± 112.42 pg/mL, which was significantly higher than that in the remission group (105.96 ± 64.79 pg/mL) (p < 0.01 ) and control group (56.03 ± 14.49 pg/mL) (p < 0.001 ). That in the remission group was also significantly higher than that in the control group (p < 0.01 ). They were negatively correlated with hemoglobin, red blood cell, white blood cell, and platelet counts and positively correlated with the percentage of CD19 + B cells and CD5 + CD19 + /CD19 + B cells, respectively. The mRNA expression levels of PU.1 and BATF in IRP patients were higher than those in controls (p < 0.05 ). Conclusions . The percentage of Th9 cells in the peripheral blood and the level of IL-9 in the serum of patients with IRP were increased, which was related to the severity of the disease.