Inactivation of H‐type and L‐type bovine spongiform encephalopathy following recommended autoclave decontamination procedures. Issue 5 (8th March 2020)

• Record Type: Journal Article
• Authors: Chapman, Gail E.; Lockey, Richard; Beck, Katy E.; Vickery, Chris; Arnold, Mark; Thorne, Leigh; Thorne, Jemma K.; Walker, Sarah R.; van Keulen, Lucien; Casalone, Cristina; Griffiths, Peter C.; Simmons, Marion M.; Terry, Linda A.; Spiropoulos, John
• Is Part Of: Transboundary and emerging diseases. Volume 67:Issue 5(2020)
• Page Start: 1872
• Page End: 1878
• Publication Date: 2020-03-08
• Subjects: bovine spongiform encephalopathy -- H‐type -- inactivation -- L‐type -- transmissible spongiform encephalopathie; Veterinary medicine -- Periodicals; 636.089
• DOI: 10.1111/tbed.13513
• Languages: English
• ISSNs: 1865-1674

Abstract

Abstract: The resistance of H‐type and L‐type BSE prions to autoclaving under EU regulation conditions for specified risk material is unknown. We employed transgenic mouse (bovinized line tg1896) bioassay to assess the efficacy of such decontamination on L‐ and H‐type BSE. For each source, titre calculation was based on the comparison of incubation period and attack rate prior to and after decontamination. The infectious titre of L‐type BSE was reduced by at least 9.40 log10 and of H‐type BSE by at least 3.94 log10 . In fact, no infectivity was detected for L‐type or H‐type BSE post‐inactivation even at a 10 –1 dilution.