Weaning affects the glycosidase activity towards phenolic glycosides in the gut of piglets. Issue 5 (25th April 2020)

• Record Type: Journal Article
• Authors: Van Noten, Noémie; Van Liefferinge, Elout; Degroote, Jeroen; De Smet, Stefaan; Desmet, Tom; Michiels, Joris
• Is Part Of: Journal of animal physiology and animal nutrition. Volume 104:Issue 5(2020)
• Page Start: 1432
• Page End: 1443
• Publication Date: 2020-04-25
• Subjects: enzymes -- glycosidase -- phenolic glycosides -- piglets -- weaning; Animal nutrition -- Periodicals; Feeds -- Periodicals; 636.085
• DOI: 10.1111/jpn.13368
• Languages: English
• ISSNs: 0931-2439

Abstract

Abstract: Phenolic compounds in pig diets, originating either from feed ingredients or additives, may occur as glycosides, that is conjugated to sugar moieties. Upon ingestion, their bioavailability and functionality depend on hydrolysis of the glycosidic bond by endogenous or microbial glycosidases. Hence, it is essential to map the glycosidase activities towards phenolic glycosides present along gut. Therefore, the activity of three key glycosidases, that is α‐glucosidase (αGLU), β‐glucosidase (βGLU) and β‐galactosidase (βGAL), was quantified in small intestinal mucosa and digesta of piglets at different gastrointestinal sites (stomach, three parts of small intestine, caecum and colon) and at different ages around weaning (10 days before and 0, 2, 5, 14 and 28 days after weaning). Activity assays were performed with p ‐nitrophenyl glycosides at neutral pH. The αGLU activities in mucosa and digesta were low (overall means 1.4 and 60 U respectively) as compared to βGLU (15.2 and 199 U) and βGAL (23.4 and 298 U; p < .001). Moreover, αGLU activity in mucosa was unaffected by age. Conversely, βGLU and βGAL activities dropped significantly after weaning. Minimal levels, ranging between 18% and 54% of the pre‐weaning values, were reached at 5 days post‐weaning. Similarly, in small intestinal digesta, reductions from 60% up to 90% were observed for the three enzyme activities on day five post‐weaning as compared to pre‐weaning levels. In caecal contents, activities were lowest at 14 days post‐weaning, while in stomach and colon no clear weaning‐induced effects were observed. Our data suggest that weaning affects the glycosidase activity in mucosa (mainly endogenous origin) and digesta (primarily bacterial origin) with the most pronounced effects occurring 5 days post‐weaning. Moreover, differences in activities exist between different glycosidases and between gut locations. These insights can facilitate the prediction of the fate of existing and newly synthetized glycosides after oral ingestion in piglets.