Spectral and molecular docking studies of nucleic acids/protein binding interactions of a novel organometallic palladium (II) complex containing bioactive PTA ligands: Its synthesis, anticancer effects and encapsulation in albumin nanoparticles. (25th June 2020)

• Record Type: Journal Article
• Authors: Lighvan, Zohreh Mehri; Khonakdar, Hossein Ali; Heydari, Abolfazl; Rafiee, Mina; Jahromi, Maryam Dehdashti; Derakhshani, Ali; Momtazi‐Borojeni, Amir Abbas
• Is Part Of: Applied organometallic chemistry. Volume 34:Number 10(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-06-25
• Subjects: albumin nanoparticle -- BSA binding -- DNA binding -- palladium complex -- site markers; Organometallic chemistry -- Periodicals; Organometallic compounds -- Periodicals; 547.05
• DOI: 10.1002/aoc.5839
• Languages: English
• ISSNs: 0268-2605

Abstract

Abstract : The organometallic palladium complex with nitrogen‐containing heterocycles is a potent antitumor agent. Coordination of phosphorus ligands to organometallic complexes increases their hydrophilicity, promotes ligand−DNA interactions and damage level to cancer cells, and blocks division in target cells. In this study, a phosphaadamantane palladium complex ([Pd{(C, N)‐ (C12 H8 NH2 )} (PTA) Cl], PTA = 1, 3, 5‐Triaza‐7‐phosphaadamantane) (2 ) was synthesized via the reaction of biologically active PTA with binuclear palladacycles [Pd2 {(C, N)‐(C12 H8 NH2 )}2 (μ‐Cl)2 ] (1 ). In vitro studies of the complex with DNA (calf‐thymus) explored by UV–Vis, emission titration, circular dichroism and helix melting methods showed that the complex interacts with DNA via an intercalative mechanism. Furthermore, competitive binding studies using warfarin, digoxin and ibuprofen site markers containing definite binding sites revealed the binding of the complex to site I on bovine serum albumin. The in vitro release mechanism of the palladium complex exhibited a biphasic pattern characterized by an initial burst release followed by a slower sustained release. Ultimately, in vitro evaluation of cytotoxicity and cell death showed that the complexes were able to decrease the viability of human cancer cell lines (MCF‐7 and Jurkat) in a dose‐dependent manner, but lower decreases were observed in the viability of normal fibroblast cells ASF‐4 at the dosages evaluated. Finally, the order of in vitro anticancer activities was found to be consistent with the DNA‐binding affinities. Abstract : Synthesis, spectral and molecular docking studies on the nucleic acids/protein binding interactions of new organometallic palladium (II) complex containing bioactive PTA ligand, anticancer effects and its encapsulation in albumin nanoparticle.