CTAB‐PLGA Curcumin Nanoparticles: Preparation, Biophysical Characterization and Their Enhanced Antifungal Activity against Phytopathogenic Fungus Pythium ultimum. Issue 34 (10th September 2020)

• Record Type: Journal Article
• Authors: Khatua, Ashapurna; Prasad, Abhinav; Priyadarshini, Eepsita; Virmani, Ishita; Ghosh, llora; Paul, Bernard; Meena, Ramovatar; Barabadi, Hamed; Patel, Amiya Kumar; Saravanan, Muthupandian
• Is Part Of: ChemistrySelect. Volume 5:Issue 34(2020)
• Page Start: 10574
• Page End: 10580
• Publication Date: 2020-09-10
• Subjects: Antifungal agents -- Curcumin nanoparticles -- Fluorescent probes -- Pythium ultimum -- Reactive oxygen species; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/slct.202002158
• Languages: English
• ISSNs: 2365-6549

Abstract

Abstract: In the current study, we utilized application of nanotechnological advancements to synthesize positively charged curcumin nanoparticles (CurNPs). In CurNPs, curcumin (Cur) was encapsulated by a stabilizer, polymer poly(lactic‐co‐glycolic acid) (PLGA) and its surface charge was modified by cationic surfactant cethyltrimetylammonium bromide (CTAB). Characterization methods involving UV‐visible spectroscopy, X‐ray diffraction (XRD), transmission electron microscopy (TEM) and dynamic light scattering (DLS) were employed to confirm their synthesis. Then, we used CurNPs to investigate their potential as fungicidal agents in vitro and the underlying mechanisms, as compared to free Cur in the two fungal strains SR1 and BP1120 of a destructive plant pathogen Pythium ultimum var . ultimum . The fungicidal activity of CurNPs were studied by several methods which resulted in comparatively more pronounced antifungal activity in BP1120 than SR1. Broth dilution and well diffusion assay revealed minimum inhibitory concentration (MIC80 ) for CurNPs to be 52.57 μg/mL and 44.67 μg/mL and an increase in zone of inhibition (ZOI) by 5.4 and 6.3 fold of Cur at 15 μg/mL of CurNPs in SR1 and BP1120, respectively. Study of growth curve showed prolonged lag phase, delayed and short log phase, early and prolonged stationary phase and early decline phase after CurNPs exposure. Toxicity of CurNPs in SR1 and BP1120 strains of P. ultimum was attributed to the enhancement in intracellular reactive oxygen species (ROS) generation and fall in mitochondrial membrane potential (MMP) as revealed by spectrofluorometric assay. Taken together, these CurNPs were confirmed as a novel and very potent fungicidal agents against P. ultimum var . ultimum with a great promise of controlling and treating other microbial infections. Abstract : Curcumin nanoparticles (CurNPs) were synthesized, where curcumin was entrapped inside polymer poly(lactic‐co‐glycolic acid) (PLGA) and cethyltrimetylammonium bromide (CTAB), a cationic surfactant was used to provide positive surface charge to the nanoparticles. CurNPs were employed to explore their potential as antifungal agents compared to free curcumin against a destructive phytopathogenic fungus Pythium ultimum . var . ultimum . The sensitivity of CurNPs as antifungal agents was found to be strain‐dependent and BP1120 strain exhibited superior antifungal activity than SR1 strain.