Expression of interleukin (IL)‐10 family cytokines in aqueous humour of patients with specific endogenous uveitic entities: elevated levels of IL‐19 in human leucocyte antigen‐B27‐associated uveitis. Issue 5 (13th February 2019)

• Record Type: Journal Article
• Authors: Abu El‐Asrar, Ahmed M.; Berghmans, Nele; Al‐Obeidan, Saleh A.; Gikandi, Priscilla W.; Opdenakker, Ghislain; Van Damme, Jo; Struyf, Sofie
• Is Part Of: Acta ophthalmologica. Volume 97:Issue 5(2019)
• Page Start: e780
• Page End: e784
• Publication Date: 2019-02-13
• Subjects: aqueous humour -- interleukin‐10 family cytokines -- interleukin‐19 -- uveitis; Ophthalmology -- Periodicals; 617.7005
• DOI: 10.1111/aos.14039
• Languages: English
• ISSNs: 1755-375X

Abstract

Abstract: Purpose: Evidence exists that the interleukin (IL)‐10 family of cytokines is involved in autoimmune diseases. The aim of this study was to analyse the levels of the IL‐10 family cytokines IL‐10, IL‐19, IL‐20, IL‐22, IL‐26, IL‐28A and IL‐29 in aqueous humour (AH) samples from patients with specific uveitic entities. In addition, we correlated their levels with the levels of the proinflammatory cytokines tumour necrosis factor‐ α (TNF‐ α ) and IL‐1 β . Methods: Aqueous humour (AH) samples from patients with active uveitis associated with Behçet's disease (BD; n = 13), sarcoidosis ( n = 8), human leucocyte antigen (HLA)‐B27‐related inflammation ( n = 12), Vogt–Koyanagi–Harada (VKH) disease ( n = 12) and control subjects ( n = 9) were assayed with the use of a multiplex assay. Results: Of all the IL‐10 family cytokines studied, only IL‐19 levels were significantly higher in AH samples of patients ( n = 45) than in controls (p = 0.022). When comparing the four individual disease groups to controls, IL‐19 levels were only significantly higher in HLA‐B27‐associated uveitis (p < 0.001). IL‐19 levels were significantly higher in patients with HLA‐B27‐associated uveitis than in patients with BD, sarcoidosis and VKH disease (p < 0.001; p = 0.002; p < 0.001, respectively). Significant correlations were found between AH levels of IL‐19 and AH levels of TNF‐ α, ( r = 0.3; p = 0.03) and IL‐1 β ( r = 0.56; p < 0.001). Conclusions: Among the IL‐10 family cytokines analysed, IL‐19 demonstrated the highest expression in endogenous uveitis, particularly in HLA‐B27‐associated uveitis. IL‐19 thus might assist in the regulation of inflammation in HLA‐B27‐associated uveitis.