Polyphenol Characterization, Antioxidant and Skin Whitening Properties of Alnus cordata Stem Bark. Issue 9 (28th August 2019)

• Record Type: Journal Article
• Authors: Smeriglio, Antonella; D'Angelo, Valeria; Denaro, Marcella; Trombetta, Domenico; Raimondo, Francesco Maria; Germanò, Maria Paola
• Is Part Of: Chemistry & biodiversity. Volume 16:Issue 9(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-08-28
• Subjects: Alnus cordata -- Betulaceae -- mushroom tyrosinase -- zebrafish -- polyphenols -- RP-LC-DAD analysis -- antimelanogenic activity; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Biodiversity -- Periodicals; 572
• DOI: 10.1002/cbdv.201900314
• Languages: English
• ISSNs: 1612-1872

Abstract

Abstract: In this study, we investigated the phenolic composition of the crude extract (MeOH 80 %) of Alnus cordata (Loisel .) Duby stem bark (ACE) and its antioxidant and skin whitening properties. RP‐LC‐DAD analysis showed a high content of hydroxycinnamic acids (47.64 %), flavanones (26.74 %) and diarylheptanoids (17.69 %). Furthermore, ACE exhibited a dose‐dependent antioxidant and free‐radical scavenging activity, expressed as half‐maximal inhibitory concentration (IC50 ): Oxygen radical absorbance capacity (ORAC, IC50 1.78 μg mL −1 )>Trolox equivalent antioxidant capacity (TEAC, IC50 3.47 μg mL −1 )>2, 2‐Diphenyl‐1‐picrylhydrazyl (DPPH, IC50 5.83 μg mL −1 )> β ‐carotene bleaching (IC50 11.58 μg mL −1 )>Ferric reducing antioxidant power (FRAP, IC50 17.28 μg mL −1 ). Moreover, ACE was able to inhibit in vitro tyrosinase activity (IC50 77.44 μg mL −1 ), l ‐DOPA auto‐oxidation (IC50 39.58 μg mL −1 ) and in an in vivo model it exhibited bleaching effects on the pigmentation of zebrafish embryos (72 h post fertilization) without affecting their development and survival. In conclusion, results show that A. cordata stem bark may be considered a potential source of agents for the treatment of skin disorders due to its bleaching properties and favorable safety profiles, associated to a good antioxidant power. Abstract :