Fluorogenic 7-azidocoumarin and 3/4-azidophthalimide derivatives as indicators of reductase activity in microorganisms. Issue 16 (15th August 2019)

• Record Type: Journal Article
• Authors: Chalansonnet, Valerie; Lowe, John; Orenga, Sylvain; Perry, John D.; Robinson, Shaun N.; Stanforth, Stephen P.; Sykes, Hannah E.; Truong, Thang V.
• Is Part Of: Bioorganic & medicinal chemistry letters. Volume 29:Issue 16(2019)
• Page Start: 2354
• Page End: 2357
• Publication Date: 2019-08-15
• Subjects: Fluorogenic substrates -- Fluorogenic azides -- Nitroreductase -- Microorganism detection; Bioorganic chemistry -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1016/j.bmcl.2019.06.010
• Languages: English
• ISSNs: 0960-894X

Abstract

Graphical abstract: Abstract: A series of fluorogenic heterocyclic azides were prepared and assessed as reductase substrates across a selection of Gram-negative and Gram-positive microorganisms. The majority of these azides showed similar activity profiles to nitroreductase substrates. Microorganisms that do not produce hydrogen sulfide reduced the azides, indicating reductase activity was not linked to hydrogen sulfide production.